针刺联合中药治疗外伤性视神经病变兔的实验研究

Experimental Study of Acupuncture Combined with Traditional Chinese Medicine on the Treatment of Traumatic Optic Neuropathy Rabbits

目的观察兔视神经损伤后分别采用中药、针刺、中药联合针刺治疗后闪光视觉诱发电位(flash visual evoked potentials,F—VEP)的改变,为指导临床治疗提供参考。方法选择15只新西兰大耳白兔施行左眼改良Krolein手术,钳扎视神经的方法建造外伤性视神经病变模型,于造模成功后随机分3组采用中药、针刺、中药联合针刺治疗,记录造模前及造模后第1天和第7天VEP的变化。观察视神经损伤的眼底改变。结果造模成功后15只眼均表现为振幅降低,潜伏期延长。视神经损伤1周后三个组兔左眼P波潜伏期均不同程度的延长,其中针药治疗组潜伏期最短,中药对照组、针刺对照组、针药联合组之间两两比较差异均有统计学意义(P<0.05)。三个组治疗后兔眼P波振幅均有不同程度升高,其中针药治疗组振幅升高最明显,三个组治疗前后振幅差异均有统计学意义(P<0.05),中药对照组、针刺对照组、针药联合组之间两两比较差异均有统计学意义(P<0.05)。结论中药及针刺治疗兔外伤性视神经病变有一定的疗效,联合治疗优于单一治疗,可以进行临床治疗的相关研究。

Objective： To observe the changes of flash visual evoked potentials （F -VEP） of optic nerve injury rabbits after they were treated with acupuncture, traditional Chinese medicine, and combination of acupuncture and traditional Chinese medicine and provide ref- erence for clinical treatment. Methods： 15 New Zealand white rabbits were conducted with Krolein surgery on their left eyes and pliers tie method was used to make traumatic optic neuropathy models. After the success in the modeling, the rabbits were randomly divided into three groups, respectively treated with acupuncture, traditional Chinese medicine, and combination of acupuncture and traditional Chinese medicine. The changes of VEP were recorded on the first day and the seventh day after modeling to observe their ocular fundus changes. Results： After modeling, 15 eyes showed decrease amplitude and extension latency. After one -week optic nerve injury, the P- wave la- tency of the left eyes of the three groups extended at varying degrees, among which the latency of the acupuncture and medicine group was shortest. The comparison of the TCM control group, acupuncture group and acupuncture - Chinese medicine combination group showed the differences had statistical significance （ P 〈 0. 05 ）. The P - wave amplitude of the left eyes of the three groups increased to varying degree after the treatment, in which the amplitude of the acupuncture and medicine group increased most obviously. The amplitude differences of the three groups were statistically significant （P 〈 0. 05 ） before and after the treatment. The comparison of the TCM control group, acu- puncture group and acupuncture - Chinese medicine combination group showed the differences had statistical significance （ P 〈 0.05 ）. Conclusion ： The TCM and acupuncture have a certain effect on the treatment of traumatic optic neuropathy rabbits, and the acupuncture - Chinese medicine combination therapy is superior to monotherapy and can be applied in clinical treatment.