原发性肝癌患者血清肿瘤标志物与肿瘤组织中凋亡调节基因表达的关系

Relationship between serum tumor marker level with apoptosis regulation gene expression of tumor tissue in patients with primary hepatocarcinoma

目的探讨原发性肝癌患者血清肿瘤标志物与肿瘤组织中凋亡调节基因表达的关系。方法 40例原发性肝癌患者与40例健康者分别纳入观察组和对照组,检测2组受试者的高尔基体蛋白73(GP73)、胸苷激酶(TK1)、Dickkopf1(DKK1)水平,以及肿瘤组织中凋亡调节基因的表达水平。结果观察组患者血清中GP73、TK1、DKK1水平明显高于对照组,差异有统计学意义(P<0.05)。肝癌组织中凋亡抑制基因Plk1、Livin和Xiap的水平高于癌旁正常组织,促凋亡基因MTS1、Caspase-3、Caspase-8的水平低于癌旁正常组织,差异均有统计学意义(P<0.05)。血清GP73、TK1、DKK1水平与肝脏组织中Plk1、Livin、Xiap水平呈正相关,与MTS1、Caspase-3、Caspase-8的水平呈负相关。结论原发性肝癌患者血清中GP73、TK1、DKK1水平异常升高,且与肿瘤组织中凋亡调节基因的表达密切相关,是判断原发性肝癌病情的理想指标。

Objective To study the relationship between serum tumor marker level and the apoptosis regulation gene of tumor tissue in the patients with primary hepatocarcinoma. Methods 40 cases of primary hepatocarcinoma and 40 healthy people were in- cluded into the observation group and control group. Then tbe levels of tumor marker GP73 ,TK1 ,DKK1 in serum and the expres- sion of apoptosis regulation gene in tumor tissue were detected in the two groups. Results The serum GP73,TK1 and DKK1 levels of the observation group were significantly higher than those of the control group, the difference was statistically significant（P〈0.05）. The apoptosis inhibiting gene Plkl ,Livin and Xiap levels in the hepatocellular carcinoma tissue were higher than those in the adjacent normal tissues,while the pro-apoptotic gene MTS1 ,Caspase 3 and Caspase-8 levels were lower than those in the adjacent normal tissues,the difference had statistical significance（P〈0.05）; serum GP73, TK1 and DKK1 levels were positively correlated with Plkl, Livin and Xiap levels and negatively correlated with MTS1, Caspase-3 and Caspase-8 levels. Conclusion The levels of serum GP73,TK1 and DKK1 in the patients with primary hepatocarcinoma are abnormally increased,moreover which are closely correlated with the apoptosis regulating gene expression and the ideal indexes to evaluate the disease condition of primary hepatocarci- noma.