肝素锂对比浊法检测微量总蛋白的干扰分析

The analysis of heparin lithium interference in turbidimetric method to detect total protein

目的证实肝素锂对比浊法检测微量总蛋白的干扰效应,并对其进行相关方法性能评价。方法分别配对检测8份不同浓度微量总蛋白尿液干扰标本,筛查判断肝素锂是否是试验的干扰物;浓度0.172、0.427g/L微量蛋白质标本中分别加入不同量的肝素锂（干扰物试验系列样品,肝素锂浓度分别为0.000、0.025、0.030、0.035、0.040、0.045、0.050mg/L）,重复检测4次,进行干扰物剂量效应试验。结果干扰标本检测结果显示,8份含肝素锂与不含肝素锂的配对标本中最大偏倚为159.37%,最小偏倚为11.37%,2组间结果差异有统计学意义（t=17.24,P〈0.05）;浓度为0.025mg/L肝素锂对0.172g/L微量蛋白质标本干扰效果的95%置信区间为0.034-0.062;0.030mg/L肝素锂对0.427g/L干扰效果的95%置信区间为0.043-0.053;肝素锂浓度分别为0.025mg/mL和0.050mg/mL时,0.172g/L微量蛋白质标本的结果偏倚分别为27.33%、58.14%;肝素锂浓度分别为0.030mg/mL和0.050mg/mL时,0.427g/L微量蛋白质标本的结果偏倚分别为11.24%、18.74%;同系列浓度的肝素锂对0.172g/L和0.427g/L微量蛋白质标本的干扰物剂量呈线性相关,其线性方程分别为Y=1.974 X-0.001 03,Y=1.599 X-0.000 5。结论肝素锂是比浊法检测微量蛋白质的外源性干扰物,它对微量蛋白质检测结果为正干扰,特别对低水平微量蛋白质检测的干扰效果更明显,且随着肝素锂浓度升高,干扰效果越强。

Objective To confirm the interference effect of heparin lithium on turbidimetry method for detecting total protein, and to conduct performance evaluation of it. Methods Eight different concentrations of total protein urine specimen with and with- out heparin lithium were detected respectively, to confirm the interference of heparin lithium on the experiment. Adding different concentration of heparin lithium solution to the prepared total protein samples with concentration of 0. 172 ,0. 427 g/L（a series of interference experiment samples,with heparin lithium concentrations of 0. 000,0. 025,0. 030,0. 035,0. 040,0. 045,0. 050 mg/L）, repeat four times detection,and then conduct the dose effect experiment of interference. Results The biggest bias of the testing re suhs in the two paired samples was 159.37%,the mini bias was 11.37G ,the difference of the results in two groups was statistical significant（t=17.24 ,P〈0.05）. 95% confidence interval of the interference effect in the total protein samples with concentration of 0. 172 g/L was 0. 034-0. 062, while in the total protein samples with concentration of 0. 427 g/L was 0. 043-0. 053. When the beparin lithium were 0. 025 mg/mL and 0. 050 mg/mL respectively,the results of bias of the total protein samples with concentra tion of 0. 172 g/L were 27. 33%, 58. 14% respectively. But in the 0. 427 g/L total protein samples, the results of bias were 11. 24%,18. 74% respectively. Dose effect of interference both showed a linear relationship in the series concentration of the hepatin lithium and interference of sample of 0. 172 g/L and 0. 427 g/L,and the linear equations were Y=1.974X-0. 001 03,Y=1. 599 X=0. 000 5 respectively. Conclusion Heparin lithium is the exogenous interfering substance to the detection of total protein in turbidimetry method,it is the positive interference to the determination results of total proteins, especially for the low level of total pro tein detection,and as heparin lithium concentration increases, the stronger the interference effect.