乳酸在DH5α、W3110菌株中的发酵生产的研究

The Research of Lactic Acid Production by Fermenting in the Strain DH5α and the Strain W3110

利用PCR技术从Lactococcus lactis基因组中获得1kb的编码L-乳酸脱氢酶的基因l-ldh,通过T4连接酶将其连接到质粒p Bluescript SK(-)。构建好的重组质粒p SK(l-ldh)分别转化Eschericbia coli DH5α、W3110,以葡萄糖为唯一碳源发酵生产DL-乳酸或L-乳酸,并检测了OD550、细胞湿重。

The L-lactate dehydrogenase encoding gene 1-1dh from Lactococcus lactis was amplified by PCR. L-ldh was inserted in pBluescript SK（-） to yield the recombinant vector pSK（l-ldh）. The recombinant vector pSK（l-ldh） was introduced into DH5α. W3110 to express the gene 1-1dh. Fermentation of DL-lactic acid or L-lactic acid was achieved with glucose as role carbon source. Optical density at 550 nm. moist cell weight and the account of DL- lactic acid or L-lactic acid was measured.