复发性阿弗他溃疡自身免疫的分子模拟机制

Recurrent aphthous ulceration and molecular mimicry

目的：本研究旨在探查复发性阿弗他溃疡（RAU ）患者 T 细胞识别热休克蛋白（HSP）的抗原表位。方法采用从结核分枝杆菌65kD HSP 获得的小肽段刺激外周血单个核细胞，做淋巴细胞增殖试验确定 T 细胞表位。结果 RAU 患者只对65kD HSP91‐105肽段起明显的特异性淋巴细胞增殖反应（P ＜0．01）；对人类同源性60kD HSP91‐130肽段的对比研究表明 RAU 患者对照组有更显著的淋巴细胞增殖反应（P＜0．01）。结论本研究提示 RAU 可能由口腔微生物 HSP91‐105肽段刺激口腔黏膜郎罕氏细胞所启动，通过分子模拟使针对同源性HSP116‐130肽段的自身反应性 T 细胞克隆增殖。

Objective The aim of this study was to investigate T cell epitopes of the 65 kD heat shock protein （HSP）in patients with recurrent aphthous ulcers（RAU） .Methods Peripheral blood mononuclear cells were stimula‐ted from 60 kD of homologous human HSP .Results Specific lymphoproliferative responses were stimulated only with peptide 91‐105 in RAU ,compared with healthy or disease control（P〈 0 .01） .A comparative investigation with the homologous human 60‐kD hsp 116‐120 also showed significantly greater lymphoproliferative responses in RAU than in healthy or disease controls（P〈 0 .01） .Conclusion The results suggest that oral ulceration might be initiated by the microbial hsp peptide 91‐105 stimulating the mucosal Langerhans cells ,which may generate autoreactive T cell clones primed to the human homlolgous HSP peptide 116‐130 .