疏肝化痰祛瘀方对乳腺癌细胞增殖能力调节的机制研究

Mechanism of Shuganhuatanquyufang on Regulating MCF- 7 Cell Proliferation

目的观察疏肝化痰祛瘀方含药血清对人乳腺癌细胞MCF-7增殖能力的调节。方法应用疏肝化痰祛瘀方中药汤剂给予大鼠灌胃,取大鼠腹主动脉血制备含药血清。将人乳腺癌细胞MCF-7在体外进行传代培养,分3组进行干预其生长,分别为空白组、对照组(加入表阿霉素)、实验组(加入疏肝化痰祛瘀方含药血清)。3组分别培养24、48及72h,检测细胞增殖及凋亡情况,对比各组不同作用时间细胞增殖抑制率及凋亡率。结果疏肝化痰祛瘀方对MCF-7细胞体外增殖具有一定的抑制作用,随着作用时间的延长,抑制作用逐渐增强(P<0.05),与表阿霉素组作用相似;疏肝化痰祛瘀方可诱导MCF-7细胞凋亡,与作用时间呈正相关(P<0.05)。结论疏肝化痰祛瘀方含药血清具有抑制人乳腺癌细胞MCF-7增殖与诱导其凋亡的作用,与作用时间呈正相关,提示该方剂发挥临床治疗乳腺癌的疗效机制所在。

Objective To investigate the mechanism of Shuganhuatanquyufang on regulating human adenocarcinoma cell （ MCF - 7） proliferation. Methods Serum containing drug was obtained from the blood achieved in rodent aorta abdominalis after Shuganhuatanquyu- fang gavage. In vitro MCF -7 cell were separated into control group, positive control group （with pharmorubicin） and drug treated group （ with Serum containing Shuganhuatanquyufang）. After 24, 48, 72h the cell proliferation and apoptosis ratio were examined. Results The suppressive effect on MCF - 7 proliferation played a time depended manner（ P 〈 0.05 ）. The condition was similar in positive control group and drug treated group. The MCF -7 cell apoptosis ratio in drug treated group also played a time depended manner（ P 〈 0.05 ）. Conclusion Serum containing Shuganhuatanquyufang drug could suppress MCF -7 cell proliferation and induce apoptosis in a time depended manner, which might be the possible mechanism of this prescription on treating human adenocarcinoma in clinic.