p53和鼠双微粒体2在肾母细胞瘤中的表达

Expression of p53 and murine double minute 2 in nephroblastoma

目的探讨p53和鼠双微粒体2(murine double minute 2,MDM2)在肾母细胞瘤中的表达,以及其在疾病发生、发展中的作用。方法 36例肾母细胞瘤患者的手术组织标本36份为肾母细胞瘤组,以相应瘤旁正常肾组织标本12份为对照组。采用免疫组织化学EliVision法检测2组P53蛋白和MDM2蛋白表达情况;比较不同临床分期、预后、病理类型肾母细胞瘤P53和MDM2蛋白表达差异,分析MDM2与p53的相关性。结果肾母细胞瘤组P53蛋白阳性表达率(30.6%)及MDM2蛋白阳性表达率(41.7%)高于对照组(0、8.3%)(P<0.05);预后良好型肾母细胞瘤P53蛋白和MDM2蛋白阳性率分别为15.4%、23.1%,低于预后不良型(70.0%、90.0%)(P<0.05);P53和MDM2蛋白在临床Ⅰ期、Ⅱ期肾母细胞瘤及4个不同组织学分型肾母细胞瘤的表达差异无统计学意义(P>0.05);肾母细胞瘤中MDM2蛋白与P53蛋白表达呈正相关(c=0.663,P=0.000)。结论 P53蛋白及MDM2蛋白阳性表达可用于判断肾母细胞瘤细胞分化程度,但不能作为临床分期的参考指标;p53和MDM2阳性表达与肾母细胞瘤的发生、发展有关。

Objective To investigate the expressions of p53 and murine double minute 2 （MDM2） in nephroblastoma and their roles in the occurrence and development. Methods The expressions of P53 and MDM2 proteins were detected by EliVision method in 36 specimens of nephroblastoma tissue （nephroblastoma group） and 12 specimens of adjacent normal tissue （control group） from patients with nephroblastoma. The different expressions of P53 and MDM2 protein were compared in clinical stages, prognosis and pathologic types between two groups, and the correlation of MDM2 and p53 was analyzed. Results The positive expression rates of P53 and MDM2 proteins were significantly higher in nephroblastomagroup （30.6%, 41.7%） than those in control group （0, 8. 3%） （P〈0.05）, and lower in those with good prognosis （15. 4%, 23. 1%） than those with poor prognosis （70. 0%, 90. 0%） （P〈0.05）. There were no significant differences in the expressions of P53 and MDM2 proteins in clinical stage I and II , as well as in four pathologic types of nephroblastoma （P〉0.05）. The expression of MDM2 was positively correlated with P53 （c=0. 663, P=0. 000）. Conclusion The positive expressions of P53 and MDM2 proteins can be used to judge cell differentiation degrees but not be used as reference indicators for the clinical staging of nephroblastoma. The positive expressions of p53 and MDM2 are correlated with the occurrence and development of nephroblastorna.