血脂康对肥胖大鼠肝脏SOCS-3 mRNA表达的影响

Influence of Xuezhikang on expression of suppressor of cytokine signaling 3 mRNA in obese rat liver

目的分析血脂康对肥胖大鼠肝脏SOCS-3 mRNA表达水平的影响,探讨血脂康调节肥胖大鼠糖脂代谢的作用机制。方法应用SD大鼠制作肥胖大鼠模型,将造模成功大鼠分为模型对照组和血脂康低剂量组、中剂量组、高剂量组,每组10只,并选择10只基础对照组小鼠作为空白对照组。按照既往文献,除空白对照组外均给予高脂饲料,低剂量组给予血脂康0.23 g/kg,中剂量组给予0.69 g/kg,高剂量组给予1.15 g/kg,模型对照组及空白对照组同体积水,检测各组大鼠血清胰岛素、瘦素、血糖及C肽水平及肝脏SOCS-3 mRNA。结果通过对各组大鼠血清胰岛素、血糖、C肽及瘦素水平进行比较,显示模型对照组较空白对照组各指标显著升高(P<0.05),随着血脂康给予剂量的增加,血清胰岛素、血糖、C肽及瘦素水平显著降低(P<0.05)。检测大鼠肝脏组织中SOCS-3表达水平,模型对照组较空白对照组肝脏中SOCS-3的表达显著升高(P<0.05),随着血脂康剂量的增加,大鼠肝脏中SOCS-3 mRNA的相对表达量显著降低(P<0.05)。肝脏SOCS-3 mRNA与血清瘦素间存在中度负相关性(r=-0.612,P<0.05),与血清胰岛素存在显著性负相关关系(r=-0.726,P<0.05)。结论血脂康能够通过调节体内SOCS-3 mRNA的表达,有效改善体内血清胰岛素、瘦素、血糖、C肽的表达,改善糖脂紊乱,为血脂康的临床应用提供实验基础。

Objective To analyze the impact of Xuezhikang on suppressor of cytokine signaling 3 （ SOCS-3 ） mRNA ex- pression levels in obese rats liver, and explore theregulatory mechanism of Xuezhikang on glucose and lipid metabolism. Methods The obese rats model were established and divided into model group,low dose Xuezhikang group （Xuezhikang 0.23 g/kg ）,middle dose Xuezhikang group （Xuezhikang 0.69 g/kg） and high dose Xuezhikang group （Xuezhikang 1.15 g/kg） ,with 10 rats in each group, all the rats in the four groups were administrated with high-fat foods;10 rats to which no Xuezhikang was applied were selected as null group and were administrated with conventional food. The rats in null group and model group received the same volume of water as Xuezhikang. The levels of serum insulin,leptin, glucose, C-peptide and liver SOCS-3 mRNA were detected. Results The levels of serum insulin, leptin, glucose and C-peptide in the model group were significantly higher as compared with the null group（ P 〈 0.05 ）, but they were decreased by Xuezhi- kang treatment （ positively with the dose of Xuezhikang）, P 〈 0.05 ; The liver SOCS-3 expression was significantly in- creased as compared with the null group （ P 〈 0.05 ） , rat liver SOCS-3 mRNA expression was depressed by Xuezhikang treatment （ positively with the dose of Xuezhikang） ,P 〈 0.05 ;The level of liver SOCS-3 mRNA was negative correlated with serum leptin（r = -0.612） and serum insulin（r = -0.726）,P 〈0.05. Conclusion Xuezhikang can effectively decrease the levels of serum glucose, leptin, insulin and C-peptide and improve lipid disorders through regulating the ex- pression of SOCS-3 mRNA.