丹龙醒脑方对脑缺血再灌注大鼠海马区Notch信号通路相关蛋白表达的影响

Effects of Danlong Xingnao Recipe on Protein Expressions Related to Notch Signaling Pathway in Hipppcampus of Cerebral Ischemia Reperfusion Injury Model Rats

目的探讨丹龙醒脑方对脑缺血再灌注模型大鼠海马区Notch信号通路相关蛋白Notch1、Notch2及Hes1表达的影响。方法将80只雄性SD大鼠随机分为假手术组、模型组、丹龙醒脑方小剂量组(丹小组7.4 g/kg)、大剂量组(丹大组14.8 g/kg),线栓法建立局灶性脑缺血再灌注大鼠模型,再灌注7 d取缺血侧海马组织。采用免疫组织化学法、免疫印迹法检测大鼠海马区Notch1、Notch2及Hes1蛋白表达。结果与假手术组比较,各组Notch1、Notch2及Hes1蛋白表达显著升高(P<0.05,P<0.01);与模型组比较,丹小组、丹大组Notch1、Notch2及Hes1蛋白表达显著升高(P<0.05,P<0.01)。结论丹龙醒脑方能通过上调海马区Notch1、Notch2及Hes1蛋白表达水平,调节Notch信号转导通路,这可能是其促进脑缺血损伤后神经功能修复的机制之一。

Objective To investigate the effects of Danlong Xingnao recipe （DLXNR） on the expressions of Notch1, Notch2 and Hesl protein related to the Notch signaling pathway in hippcampus of cerebral ischemia-reperfusion injury model rats. Methods 80 male SD rats were randomly divided into sham-operation group, model group, Danlong Xingnao recipe small-dose group （Dan-small group 7.4 g/kg） and Danlong Xingnao recipe high-dose group （Dan-high group 14.8 g/kg）. The local cerebral ischemia /reperfusion rat model was established by intraluminal thread occlusion of the middle cerebral artery. After reperfusion for 7d, the ischemia hippocampus tissues were taken out. The Notchl, Notch2 and Hesl protein expressions in hippocampal were determined respectively by immunohistochemicstry and Western Blot method. Results Compared with the sham-operation group, the expression of Notchl, Notch2 and Hesl protein in other groups were increased obviously （P〈0.05, P〈0.01）. Compared with the model group, the expression of Notch1, Notch2 and Hesl protein in Dan-small group and Dan-high group were increased obviously （P〈0.05, P〈0.01）. Conclusion Danlong Xingnao recipe can upregulate the expressions of Norchl, Notch2 and Hesl protein, regulate the Notch signal transduction pathway. It may be one of the mechanisms to promote the repair of brain injury.