摘要
目的探讨大鼠脑挫裂伤合并硬膜下血肿模型中TNF-α及IL-1β的表达情况。方法将120只大鼠随机分为正常组60只和模型组60只。利用球囊内注水后快速解压制作脑挫裂伤合并硬膜下血肿模型,在6、12、24 h,模型组行开颅取脑组织,SP免疫组织化学检测脑组织TNF-α及IL-1β的表达。结果 6、12、24 h,模型组大鼠脑组织中TNF-α及IL-1β水平显著升高,而正常组对应的TNF-α、IL-1β表达水平则明显降低,两组比较,差异有统计学意义(P<0.05)。结论在脑挫裂伤合并硬膜下血肿模型中,TNF-α及IL-1β表达水平在伤后6、12、24 h明显增高,提示出现脑损伤、脑水肿。
Objective To explore the expression of TNF-αand IL-1β in cerebral contusion and laceration complicated with subdural hematoma in rats. Methods One hundred and twenty rats were evenly divided into normal group and model group in random.The cerebral contusion and laceration complicated with subdural hematoma model was established by rapid decompression after injecting water using sacculus.Brain tissue was taken by craniotomy at 6 h,12 h and 24 h in model group.The expression of TNF-α and IL-1β in brain tissue were detected by SP immunohistochem- istry. Results The TNF-α and IL-1β expression level in brain tissue at 6 h,12 h and 24 h in model group was greatly improved respectively,while the corresponding expression level in normal group was obviously decreased respectively, and there was a statistical difference between two groups （P〈0.05）. Conclusion In the model of cerebral contusion and laceration complicated with subdural hematoma,expression level of TNF-α and IL-1β is greatly increase after 6 h,12 h and 24 h trauma,and it indicates that cerebral injury or edema.
出处
《中国当代医药》
2016年第3期105-107,110,共4页
China Modern Medicine
