三氯生对东北林蛙蝌蚪的急慢性毒性分析

Analysis on Acute and Sub-chronic Toxicity of Triclosan to the Northeast Frog Tadpoles

采用静置驯养-暴露方法测定了东北林蛙蝌蚪在不同质量浓度梯度三氯生中的急、慢性毒性,由剂量-效应关系获得其24 h-LC50和48 h-LC50值分别为1.578 mg·L-1和1.102 mg·L-1,48 h最大无致死效应质量浓度为0.8 mg·L-1;进一步测定了蝌蚪暴露在最大无致死效应质量浓度下11 d期间体内的过氧化氢酶活性动力学变化特征。结果显示,暴露2 d时蝌蚪体内过氧化氢酶活性升高3.5%,产生了氧化胁迫作用,暴露7 d时过氧化氢酶活性升高49.9%,达到峰值,之后呈现逐渐降低趋势但仍高于正常值。鉴于三氯生对蝌蚪的慢性损害和长期毒性明显存在,其对生态环境的综合影响将不容忽视。

In this paper the stationary tested method was adopted to determine the acute and chronic toxicity of triclosan to the northeast frog tadpoles which were exposed to different concentration gradient of triclosan. The acute data were obtained by effects- dose relationship. 24h-LC50 is 1. 578mg·L- 1and 48h- LC501. 102mg·L- 1respectively. The largest no lethal effect concentration of triclosan to the tadpoles is 0. 8mg·L- 1. By kinetics test of catalase activity it can be found that the enzyme activity increased 3. 5% in 2 days,and reached the maxium value beyond the blank value with 49. 9% in 7 days to indicate the oxidative stress effect. It should be pay more attention to chronic damage,long- term toxicity and comprehensive impact on the ecological environment.