星形细胞上调基因-1下游分子miR-885-5p裸鼠肝癌转移实验

Cancer metastasis in mouse model of a novel oncogene Metadherin mediated small molecular miR-885-5p

目的研究星形细胞上调基因-1（metadherin,MTDH）调控的下游分子mi R-885-5p在调控肝癌转移中的作用。方法收集10名癌症患者的癌组织和癌旁样品,检测MTDH和mi R-885-5p表达量。分别构建mi R-885-5p过表达慢病毒质粒和随机序列的阴性对照质粒,带有荧光标签;慢病毒包装后侵染人类肝癌细胞MHCC-97H,使用流式细胞仪分选出稳定细胞系;分别以稳定表达mi R-885-5p的MHCC-97H细胞和稳定表达随机序列的MHCC-97H阴性对照细胞对裸鼠进行腹腔及肝注射,4周后使用活体荧光成像系统检测荧光素酶信号,观察肝癌细胞在裸鼠体内成瘤的动态变化过程。结果 MTDH在肝癌组织样品的表达量较癌旁样品提高2~5倍,mi R-885-5p在肝癌组织样品的表达量较癌旁样品降低40%~90%。注射表达mi R-885-5p的肝癌细胞的动物中,荧光面积和荧光强度较对照组均明显降低（50%）。结论在肝癌组织中MTDH高表达,并且可以负调控mi R-885-5p。动物实验证明,mi R-885-5p对肝癌转移起到抑制作用。

Objective To investigate the function of miR-885-5p mediated by Metadherin（MTDH） in liver cancer metastasis in vivo. Methods MTDH and miR-885-5p expression levels were detected in cancer tissues and paraneoplastic samples of 10 cancer patients. miR-885-5p over-expressed lentiviral plasmid and scrambled negative control plasmid were constructed and infected into human liver cancer cell MHCC-97 H and the stable cell lines were selected by flow cytometry. Then MHCC-97 H cell of stably expressed mi R-885-5p and negative control plasmid were injected into nude mice. After 4 weeks, Luciferase signal was detected by in vivo fluorescence imaging system, and dynamic changes of tumors in nude mice were observed. Results The expression of MTDH in liver cancer tissue sample was 2-5 times higher than that in paraneoplastic sample, while the expression of miR-885-5p in liver cancer sample was 40%- 90% lower than that in paraneoplastic sample. The fluorescence area and intensity decreased 50% after injecting hepatoma carcinoma cell expressed by miR-885-5p in experimental group when compared with control group.Conclusion MTDH shows high-expression in liver cancer samples and it can mediate mi R-885-5p. This experiment indicates that miR-885-5p plays an inhibitory role in liver cancer metastasis in vivo.