补充葡萄糖或低聚糖对动情周期抑制大鼠瘦素水平的影响

Effects of Glucose or Oligosaccharides Supplements on Serum Leptin Level of Rats with Estrogen Cycle Inhibition

目的:在不改变大鼠运动负荷、不使用药物干预的情况下,仅以补糖方式提供外源性可利用能量干预动情周期抑制大鼠,探讨能否通过补充外源性能量改善瘦素水平,进而促进动情周期抑制的缓解。方法:45只2月龄雌性SD大鼠,在运动适应两周后,随机分为5组,每组9只。分别为控制对照组(C)、动情周期抑制模型组(M)、安静恢复组(R)、低聚糖补充组(O)、葡萄糖补充组(G)。第九周干预结束后,处死取材,测定内脏脂肪含量、血清雌激素、孕激素、瘦素水平。结果:经过3周的干预:(1)干预各组的内脏脂肪含量均显著高于M组。(2)干预各组的血清瘦素浓度均显著高于M组。(3)O组、R组、G组的血清雌二醇(E_2)浓度与C组相比均无显著性差异;M组的血清孕酮(P)浓度显著低于其余4组。结论:通过对动情周期抑制大鼠补充葡萄糖或低聚糖,促进瘦素的分泌和雌激素、孕激素的合成,有效缓解大鼠的动情周期抑制。

Objective： Neither changing the exercise load nor using drug intervention, the exogenous available energy of carbohydrate is supplemented to intervene in rats with estrogen cycle inhibition so as to determine if carbohydrate supplement can improve the level of serum leptin concentration and alleviate estrogen cycle inhibition of rats. Method： Forty-five female Sprague-Dawley rats（two months old）, after two weeks＇ exercise adaptation, were randomly and averagely divided into five experimental groups： control group（C）, estrogen cycle inhibition model group（M）, quiet recovery group（R）, oligosaccharide intervention group（O） and glucose intervention group（G）. All the rats were killed at the end of 9-weeks in order to determine the visceral fat weight and the levels of serum estrogen, progesterone and leptin. Result： Three weeks＇ intervention shows the following： 1） The intervention groups＇ visceral adipose weight was significantly higher than that of group M; 2） The intervention groups＇ serum leptin concentration was higher than that of group M; 3） There was no significant difference between the serum E_2 concentration of the groups O, R and G and that of group C. The serum P concentration of group M was evidently lower than those of the other four groups.Conclusion： Adding glucose or oligosaccharides to rats with estrogen cycle inhibition may promote the secretion of leptin and synthesis of estrogen and progestin and effectively alleviate estrogen cycle inhibition of rats.