摘要
目的:研究穿心莲内酯对人前列腺癌PC-3细胞的增殖、凋亡的影响及其作用机制。方法:体外培养PC-3细胞,采用CCK-8法测定穿心莲内酯对PC-3细胞的生长抑制率,流式细胞术检测细胞凋亡率,运用荧光定量PCR法检测Bcl-2、Bax的mRNA的表达,Western blot法检测Bcl-2、Bax的蛋白表达水平。结果:20μmol/L、40μmol/L穿心莲内酯能够显著抑制PC-3细胞的增殖、促进细胞凋亡,并明显下调Bcl-2的mRNA和蛋白的表达,上调Bax的mRNA和蛋白的表达,且呈明显量效关系。结论:穿心莲内酯能够抑制PC-3细胞的增殖,促进癌细胞早期凋亡,其机制可能是通过下调Bcl-2和上调Bax的基因和蛋白的表达而实现的。
Objective： To investigate the effect of Andrographolide on the proliferation and apoptosis of prostate cancer PC-3 cells and explore apop- tosis mechanism. Methods： PC-3 cells were cultured in vitro, growth inhibition by Andrographolide was analyzed by CCK-8 assay. Flow cy- tomctry to detect apoptosis rate, using the fluorescent quantitative PCR method to detect the expression of Bcl-2, Bax mRNA, the expression of Bcl-2 and Baxl proteins were detected by Western blot. Results： Andrographolide in 20p^mol/L,40p^mol/L can significantly inhibit the proliferation and induce the apoptosis of PC-3 cells, also can significantly down-regulate the expression of bcl-2 and up-regnlate Bax in both mRNA and protein in a dose-dependent manner. Conclusion： Andrographolide can inhibit the proliferation and promote the apoptosis of PC- 3 ceils in an early stage, the mechanism probably is realized through down-regulating Bcl-2 and up-regulating Bax both in gene and protein expression
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第6期18-20,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
广东省自然科学基金项目(No:7300314)
