白藜芦醇延长脂多糖攻击小鼠存活时间并提高微血管反应性

Resveratrol improves survival and enhances microvascular reactivity in lipopolysaccharide-challenged mice

目的:观察白藜芦醇(Res)对脂多糖(LPS)攻击小鼠血管反应性的作用。方法:腹腔注射LPS(35 mg/kg)1 h后,肌肉注射白藜芦醇(30 mg/kg)或等体积的溶媒实施干预,记录存活时间;通过生物信号采集系统记录LPS攻击后6 h和12 h的平均动脉血压,同时经右侧颈静脉注射去甲肾上腺素(4.2μg/kg),以注射前后平均动脉血压的差值评价小鼠的整体升压反应;在LPS攻击后6 h留取肠系膜二级微动脉血管,制备离体微血管环,应用离体血管张力系统观察微血管对梯度去甲肾上腺素收缩反应性的变化。结果:白藜芦醇显著延长了LPS攻击小鼠的存活时间;LPS攻击引起了小鼠6 h和12 h的平均动脉血压降低、升压反应增加,但白藜芦醇对LPS攻击小鼠的平均动脉血压及其差值没有明显的作用;LPS攻击显著降低了小鼠离体微血管对去甲肾上腺素的反应性,白藜芦醇引起了血管反应曲线左移,在一定程度上提高了血管反应性。结论:白藜芦醇对腹腔注射LPS小鼠具有一定的保护作用,表现在延长存活时间、提高微血管的反应性。

To investigate the intervention effect of resveratrol （Res） on vascular reactivity in the lipopolysacchari,de （LPS） attacking mice. Methods ： After 1 h of intraperitoneal injection of LPS （35 mg/kg） , the intramuscular injection of Res （ 30 mg/kg） or isopyknic vehi-cle was performed, respectively, and the survival time was recorded. At 6 h and 12 h after LPS injection, the mean artery pressure was moni- tored with biological signal collection system, meanwhile, the difference of MAP （ A MAP） between pre-injection and pose-injection of norep- inephrine （ NE, 4.2 μg/kg） through the right jugular vein was recorded for the evaluation of pressor response. At 6 h after LPS challenge, the level two mesenteric artery was separated and harvested from each mice for the preparation of isolated vascular rings, and the vascular re- activity of contraction tension to NE was observe using the Multi Wire Myograph System. Results： The treatment of Ras significantly pro- longed the survival time of LPS attacking mice. LPS challenge decreased the MAP and increased the pressor response at 6 h and 12 h, howev- er, the treatment of Res have no obvious effect on the MAP and A MAP in mice subjected LPS injection. Meanwhile, LPS injection reduced the reactivity to NE at multiple concentrations of isolated microvessel, Res treatment lead the response curve left shift, and enhanced the vas- cular reactivity, partly. Conclusion： These current findings suggest that Res treatment plays protective effect to mice following LPS challenge through intraperitoneal injection, as follows： prolonging the survival time and enhancing the micro-vascular reactivity.