白藜芦醇对SOD1-G93A小鼠腰髓和运动皮层LC3B的影响

Effects of resveratrol on LC3B in lumbar spinal cord and motor cortex of SOD1-G93A mice

目的：研究白藜芦醇对SOD1-G93A小鼠腰髓和运动皮层LC3B的影响。方法：36只SOD1-G93A小鼠随机分为3组,对照组、溶剂组、白藜芦醇组,每组12只,至症状早期（给药20~40天）或者终末期（给药60~80天）取材,利用激光共聚焦、免疫组织化学、Western-blot技术检测小鼠腰髓和运动皮层中LC3B的表达及LC3BⅠ/Ⅱ比值变化。结果：激光共聚焦技术及免疫组织化学技术检测发现症状早期白藜芦醇组（30mg/kg）和溶剂组小鼠腰髓运动神经元及运动皮层神经元与对照组相比LC3B表达增多;Western blot技术检测发现症状早期白藜芦醇组（30mg/kg/d）小鼠腰髓和运动皮层神经元中LC3BⅠ/Ⅱ比值分别为（1.20±0.29,1.27±0.30）,与对照组相比（1.29±0.34,1.42±0.27）降低,但无统计学差异（P〈0.05）。终末期三组间小鼠腰髓和运动皮层LC3B表达及LC3BⅠ/Ⅱ比值无明显差异。结论：白藜芦醇对SOD1-G93A小鼠腰髓和运动皮层LC3B的影响微弱。

Objective： To study the influence of resveratrol on LC3B in lumbar spinal and motor cortex of SOD1 G93A mice. Methods： 36 SOD1 - G93A mice were randomly divided into control group, vehicle group and resveratrol group. Every group has 12 mice. Every mouse was treated differently until early-symptom or end-stage. According to the technology of electron microscopy confoeal microscopy and western blot , we can detect the different changes of LC3B expression and LC3B I / I/ ratio in different conditions of lumbar spinal and motor cortex. Results： Confocal laser technology and immunohistochemical technology detected, the LC3B expression increased in lumbar spinal motor neurons and motor cortex neurons of early symptoms resveratrol group （30 mg/kg/d） mice and vehicle group mice comparing with control group mice; Western blot technique detected, LC3B I / II ratio respectively isl. 20 ± 0.29 and 1.27 ± 0. 30 in lumbar spinal motor neurons and motor cortex neurons of early symptoms resveratrol group （30 mg/kg/d） mice, comparing with control goup mice 1.29 ±0.34 and 1.42 ±0.27. There is no significant difference （ P 〈 0. 05）. The LC3B expression and LC3B I / Ⅱ ratio has no obvious difference between three groups of End-stage mice （ P 〉 0.05 ）. Conclusion： The influence of resveratrol on LC3B is limited in the lumbar spinal and motor cortex of SOD1- G93A mice.