牡蛎素对正常小鼠免疫调控作用的药效学研究

The pharmacodynamics study of Ostrea talienwhanensis Crosse on the immune regulation of normal mice

目的:研究牡蛎素对小鼠免疫功能的影响,为大连湾牡蛎素的成药性评价提供药效学数据。方法:将ICR小鼠按体重随机分为对照组,贞芪扶正颗粒(阳性药)组(3.9g/kg)和牡蛎素0.0625g/kg、0.125g/kg、0.25g/kg、0.5g/kg、1.0g/kg、2.0g/kg剂量组,除对照组小鼠每天灌胃蒸馏水0.2ml/只外,其他各组小鼠每天按上述剂量灌胃0.2ml/只,15天后测定每组小鼠的各项免疫指标,即碳廓清实验检测小鼠巨噬细胞吞噬功能、MTT法检测小鼠脾淋巴细胞增殖反应、溶血空斑实验检测小鼠抗体形成细胞(B细胞)生成的能力,并且计算各组小鼠相应的免疫器官指数。结果:与对照组比较,牡蛎素0.25g/kg、0.5g/kg、1.0g/kg、2.0g/kg剂量干预后,小鼠的巨噬细胞吞噬功能显著增强(23.31%、27.75%、31.79%、27.75%)、小鼠抗体生成细胞形成的能力明显提高(17.71%、20.42%、18.50%、18.09%);牡蛎素0.0625g/kg、0.125g/kg、0.25g/kg、0.5g/kg剂量组干预后,小鼠脾淋巴细胞增殖能力显著提高(53.66%、120.73%、132.93%、123.17%、56.10%、12.20%);牡蛎素各剂量组小鼠脾脏指数、胸腺指数无显著增加。结论:不同剂量的牡蛎素对小鼠的非特异性免疫、特异性免疫(细胞免疫和体液免疫)均有相应的增强作用,随着牡蛎素剂量的增加,小鼠机体免疫不断增强,但增到一定剂量时,小鼠机体免疫不再增强,从剂量关系来看,0.5g/kg牡蛎素剂量组干预后的小鼠免疫增强作用较显著。

Objective： To study the effects of oyster on immune function in mice. This provided pharmaeodynamie data for marine traditional Chi- nese medicine Ostrea talienwhanensis Crosse in medicinal evaluation. Methods： ICR mice were randomly divided into control group, astraga- lus membranaceus-ligustrum lucidum tonic capsules （positive drug） group（3.9g/kg） and the each dose of oyster （0.0625g/kg,0. 125g/kg, 0.25g/kg,0.5g/kg, 1.0g/kg,2.0g/kg） according to the weight of the mice. Every rat received 0.2ml/day according to the above dose by o- ral gavage addition to the mice of control group receiving 0. 2ml/day distilled water separately. The immune indexes of mice of each group were determined after 15 days： the clearance rate of carborn, MTr method and hemolytic plaque experiments were used to detect the phagocy- tosis of macrophage cell, the spleen lymphocyte proliferation and the formation of antibodies generated cells （B cell） respectively in mice; more over, the immune organ indexes of mice of each group were measured. Results： Compared to control group, 0.25g/kg, 0.5g/kg, 1. 0g/kg, 2.0g/kg groups of oyster could obviously enhance the phagocytosis of macrophages （23.31%, 27.75%, 31.79%, 27.75% ） and promote the formation of antibodies generated cells in mice（ 17.71% , 20.42%, 18.50%, 18.09% ） ; 0. 0625g/kg, 0. 125g/kg, 0.25g/ kg, 0.5g/kg groups of oyster could observably promote the proliferation of lymphocyte in mice （53.66%, 120.73%, 132.93%, 123.17%, 56.10%, 12.20% ） ; the spleen index and thymus index werent improved obviously （ P 〉 0.05 ）. Conclusion： Different doses of oyster have a certain enhancement effect both nonspecific immunity and specific immunity （cellular immunity and humoral immunity）. With the increase of doses, body immunity increases in mice. But to a certain dose,the body immunity no longer increases. From the point of dose relationship, the immune enhancement effect of mice is more significant after mice receive 0.2ml/day according to 0.5g/kg dose of oyster by oral garage.