摘要
目的:观察冠脉通片对大鼠急性心肌梗死治疗作用及对心肌保护作用机制。方法:采用冠状动脉左前降支结扎术制备大鼠急性心肌梗死模型,术前预防性给药3d,1次/d,术后继续给药7d,通过死亡率、心电图、血浆肌钙蛋白T、心肌梗死重量、心肌缝隙连接蛋白Cx43、心肌胶原纤维天狼星红染色等指标,阐明冠脉通片对心肌梗死的治疗作用及作用机制。结果:冠脉通大鼠冠状动脉结扎即刻死亡率均较模型对照组低,以1.3g生药/kg组最明显,死亡率为0;冠脉通各剂量组术后即刻心电图ST段变化较模型对照组明显改善,且术后7d仍较模型对照组明显改善。冠脉通2.6、1.3g生药/kg组肌钙蛋白T明显低于模型对照组。各剂量组心肌梗死重量百分比均较模型对照组明显减少。心肌Cx染色可见模型对照组Cx表达明显减少,分布紊乱,冠脉通2.6g生药/kg组较模型对照组表达明显增多。模型对照组大鼠心肌经天狼星红染色后可见明显的、大面积红色胶原染色,冠脉通2.6g生药/kg组无1例胶原染色,1.3g生药/kg组可见胶原染色,程度低于2.6g生药/kg组,0.65g生药/kg组可见红色胶原染色,程度与模型对照组接近。结论:冠脉通预防给药后,可明显降低冠状动脉结扎即刻大鼠死亡率,改善心电图ST抬高,降低心肌梗死范围,增加心肌Cx43表达,改善心肌胶原纤维化。
Objective： To observe Guanmaitong therapeutic effects in rats with acute myocardial infarction and myocardial protective effect on the mechanism. Method ： The left anterior descending coronary artery ligation model of acute myocardial infarction was induced preoperative pro- phylac administration of 3d, 1 time/d, after continuous administration 7d. by mortalily, electrocardiogram, myocardial infarction weight, myo- caradial gap connexin Cx43, myocardial collagen fibers Sirius red staining and other indicators to clarify the therapeutic effect of myocardial in- farction and coronary artery the mechanism of particle. Result： Cx staining was significantly reduced expression of the model group, the distri- bution of disorder , coronary artery through 2.6g crude drug/kg group compared with the model group was significantly increased. Myocardial rats in the control group after Sirius red staining showed obvious, a large area of red staining of collagen. Conclusion： Guanmaitong after prophylaxis, can significantly reduce the mortality of coronary antery ligation in rats immediately improved ST-elevation myocardial infarction to reduce the scope , increased myocardial expression Cx43, Improve myocardial collagen.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第6期128-131,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
天津市中西医结合项目
