大鼠自体皮与羊皮混合移植中的COX-2、VEGF表达差异分析

THE EXPRESSION OF COX-2 AND VEGF IN MIXED GRAFTING OF SHEEPSKIN AND AUTOSKIN IN RATS

目的:通过组织学结构观察、免疫组化法检测环氧化酶-2(COX-2)、血管内皮生长因子(VEGF)在异体皮组与异种皮(羊皮组)中的表达是否存在差异,来探讨异种皮(羊皮)作为自体微粒皮肤移植的覆盖物的效果。方法:雄性健康Wistar大鼠180只,随机分为:自体皮组(对照组)、异体皮组、异种皮(羊皮)组三组,每组60只。分别于3、5、7、14、21、28d时相点每组各处死10只,应用免疫组化法检测各组各时相点COX-2、VEGF的表达,并在光镜下观察其组织学形态结构。结果:异体皮组和异种皮(羊皮)组与自体皮组(对照组)相比,大鼠创面COX-2、VEGF表达差异都有显著性(P<0.01);异种皮(羊皮)组与异体皮组大鼠创面COX-2、VEGF表达差异无显著性(P>0.05)且光镜下观察,自体皮组(对照组)在移植术后14d开始出现平行排列/新生的胶原纤维与弹力纤维,而异体皮、异种皮(羊皮)组在移植术后21d才出现平行排列/新生的胶原纤维与弹力纤维。结论:COX-2、VEGF在异体皮组与异种皮(羊皮)组的表达中差异无显著性,为临床上羊皮替代异体皮作为自体微粒皮肤移植的覆盖物提供了理论依据。

Objective：To investigate the expression of cycloxygenase-2（COX-2）,and vascular endothelial growth factor （VEGF）by using immunohistochemistry method and observe the changes of histological structure between the alloskin group and the xenoskin （sheepskin） group,and explore the effect of the xenoskin （sheepskin ）which is a kind of covering of autogenous microskin grafting.Methods：A total of 180 Wistar healthy male rats were randomly divided into 3 groups：the autoskin group （the control group ）,the alloskin group and the xenoskin group （the sheepskin group ）, with 60 rats in each group. By using immunohistochemistry method,the expression of COX-2 and VEGF were revealed on 3,5,7, 14,21 and 28d,with 10 executed rats in each group on each time points,and the structure of histology was observed by light microscope.Results：There were obvious difference of expression of COX-2 and VEGF on wound in the autoskin group compared with other two groups（P 〈0.01）;but there were no differences between the alloskin group and xenoskin group （P 〉0.05）.Observed on light microscope,the paralleled collagen and elastic fiber appeared in the autoskin group on 14 post-transplantion days,but they appeared in the alloskin group and the xenoskin group only on 21 post-transplantion days.Conclusion：There were no differences of the expression of COX-2 and VEGF in the alloskin group and the xenoskin group,which provided clinical theoretical basis on sheepskin as a substitute for the alloskin to cover autogenous microskin.