BALB/c小鼠宫颈癌原位瘤模型的建立与治疗

The Establishment and Therapy of New Cervical Tumor Model in BALB/c Mice

目的:通过建立BALB/c小鼠原位瘤模型,模拟临床肿瘤环境,为观察PTD4-apoptin融合蛋白的抗肿瘤效应提供实验基础。方法:采用两种方法建立BALB/c小鼠宫颈癌原位瘤模型,以PTD4-apoptin融合蛋白进行21天的短期治疗。TUNEL检测PTD4-apoptin融合蛋白的促凋亡效应,HE染色评估肿瘤的形态学变化。结果:与异体异位宫颈癌组织移植法相比,宫颈注射U14宫颈癌细胞悬液的成瘤效果更好。原位瘤给予PTD4-apoptin融合蛋白可显著抑制肿瘤生长,促进肿瘤细胞的凋亡。结论:小鼠宫颈注射U14宫颈癌细胞悬液可建立宫颈癌原位瘤模型,PTD4-apoptin融合蛋白可抑制该原位癌。

Objective:To establish an in situ tumor model in BALB/c mice and simulate the clinical tumor environment,providing an experimental basis for detecting the cytotoxic effect of PTD4-apoptin.Methods:On the basis of cervical cancer model,the tumors were treated with PTD4-apoptin for 21 days.TUNEL was performed to detect the cytotoxic effect of PTD4-apoptin.HE staining was used to evaluate the tumor morphological changes.Results:Two kinds of BALB/c mice with cervical cancer in situ were detected.The effect of U14 cervical carcinoma cell injection was better than heterotopic cervical cancer tissue transplants.The tumors were obviously suppressed and induced into apoptosis by PTD4-apoptin fusion protein.Conclusion:The model of cervical carcinoma in situ can be established by U14 cervical carcinoma cell injection,and PTD4-apoptin can inhibit the tumor generation.