甲基hispolon对宫颈癌U14荷瘤小鼠抗肿瘤作用的研究

Antitumor Effect of Methyl-Hispolon on Cervical Cancer U14 Tumor-Bearing Mouse

目的:探讨甲基hispolon对小鼠U14移植瘤的抑制作用,为甲基hispolon的利用提供依据。方法:细胞实验初步确定甲基hispolon对U14细胞的抑制作用,分别计算作用于U14细胞24h和48h的半抑制浓度(IC50)。小鼠腋下接种U14宫颈癌细胞,建立荷瘤小鼠模型,灌胃给药9d,观测肿瘤生长情况,计算抑瘤率;HE染色观察组织形态变化;测定肝、肾功能、血糖、血脂。结果:甲基hispolon作用U14细胞株24h的IC50值为80.9μmol/L,48h的IC50值为46.8μmol/L。甲基hispolon高剂量(15mg·kg^(-1)·d^(-1))组显著降低移植瘤体积,抑瘤率为42.26%。HE染色结果显示甲基hispolon对器官组织没有明显的毒副作用。血液生化指标分析显示,甲基hispolon可逆转因肿瘤引起的ALT和AST升高;与模型组相比,甲基hispolon高、中、低剂量组TG降低,甲基hispolon高剂量组T-CHO升高,甲基hispolon高、中剂量组血糖降低;甲基hispolon对肾功能基本没有影响。结论:甲基hispolon能显著抑制肿瘤细胞的生长,治疗剂量下对脏器组织无明显毒副作用。

Objective:To investigate the inhibitory effect of methyl-hispolon on mouse U14 xenografts and provide evidence for the utilization of methyl-hispolon.Methods:The inhibitory effect of methyl-hispolon on U14 cells was determined by cell experiments.The IC50 of methyl-hispolon on U14 cells was calculated at 24 hand 48 h,respectively.U14 cervical cancer cells were inoculated into the mouse to establish a tumor-bearing model.The mice were administered by gavage for 9days.The growth of tumor,the tumor inhibition rate and the histological changes were observed.The liver and kidney function,blood sugar and lipids were measured.Results:The IC50 value of methyl-hispolon on U14 cell line was 80.9μmol/L for 24 h,and 46.8μmol/L for 48 h.The high dose of methyl-hispolon(15 mg·kg-1·d-1)significantly reduced the tumor volume,with the tumor inhibition rate at 42.26%.HE staining showed that methyl-hispolon had no obvious toxic and side effects on organ tissues.Blood biochemical analysis showed methyl-hispolon could reverse the elevation of ALT and AST induced by tumor.Compared with the model group,TG were decreased in the high-,middle-and low-dose of methyl-hispolon group,and T-CHO was increased in the high-dose of methyl-hispolon group,and blood glucose were decreased both in the high-and middle-dose of methyl-hispolon group,with no effect on renal function.Conclusion:Methyl-hispolon can significantly inhibit tumor growth,with no obvious side effect at the therapeutic dose.