自噬相关基因Beclin-1和MAP1LC3表达与口腔癌生物学特征的关系

Relationship between autophagy-related genes Beclin-1 and MAP1LC3 expression and biological characteristics of oral cancer

目的:研究自噬相关基因Beclin-1和MAP1LC3表达与口腔癌生物学特征的关系。方法:取口腔癌组织和癌旁组织,检测Beclin-1和MAP1LC3的mRNA表达量;培养舌癌细胞株CTST-2和正常颊粘膜的原代上皮细胞,检测自噬标志分子(Beclin-1、MAP1LC3)以及促凋亡基因(P53、Caspase-3)、抗凋亡基因(生存素、Bcl-2、Bmi-1)的mRNA表达量。结果:舌癌、颊粘膜癌、牙龈癌、口底癌组织中Beclin-1、MAP1LC3的mRNA含量显著低于相应的癌旁组织;舌癌细胞CTST-2中Beclin-1、MAP1LC3的mRNA含量均低于正常粘膜细胞;舌癌细胞CTST-2中P53、Caspase-3的mRNA含量均低于正常粘膜细胞,与Beclin-1、MAP1LC3的mRNA含量呈正相关;CTST-2中生存素、Bcl-2、Bmi-1的mRNA含量均高于正常粘膜细胞,与Beclin-1、MAP1LC3的mRNA含量呈负相关。结论:口腔癌中自噬相关基因Beclin-1和MAP1LC3的表达量异常降低且与癌细胞的促凋亡基因、抗凋亡基因的表达具有显著相关性。

Objective： To study the relationship between autophagy-related genes Beclin-1 and MAP1LC3 expression and biological characteristics of oral cancer. Methods： Oral cancer tissues and precancerous tissues were collected to detect mR- NA expression levels of Beclin 1 and MAP1LC3; tongue cancer cell lines CTST-2 and primary epithelial cells of normal buccal mucosa were cultured to detect mRNA expression levels of autophagy marker molecues （Beclin-1 and MAPILC3）, pro-apopto- sis genes （P53 and Caspase-3） and anti-apoptosis genes （survivin, Bcl-2 and Bmi-1）. Results： mRNA contents of Beclin-1 and MAPILC3 in tongue cancer, buccal mucosa cancer, gingival cancer and mouth floor cancer tissues were significantly lower than those in corresponding precancerous tissues; mRNA contents of Beclin-1 and MAP1LC3 in tongue cancer cells CTST-2 were lower than those in normal mucosal cells; mRNA contents of P53 and Caspase 3 in tongue cancer cells CTST-2 were lower than those in normal mucosal cells and were positively correlated with mRNA contents of Beclin-1 and MAP1LC3; mRNA contents of survivin, Bcl-2 and Bmi-1 in CTST-2 were higher than those in normal mucosal cells and negatively correlated with mRNA contents of Beclin-1 and MAPILC3. Conclusion： Expression levels of autophagy-related genes Beclin-1 and MAPILC3 are abnormally reduced in oral cancer and are significantly correlated with the expression of pro-apoptosis genes and anti-apoptosis genes of cancer cells.