转化生长因子β激活酶1在子宫肌瘤组织中的表达及意义

Expression and significance of transforming growth factorβ-activated kinase 1 in uterine myoma tissue

目的:探讨转化生长因子β激活酶1(TAK1)在人子宫肌瘤组织中的表达及临床意义。方法:收集在本院接受手术治疗的子宫肌瘤患者76例及子宫肌瘤边界2 cm外的正常肌肉组织76例,所有患者术后的诊断均经过病理结果证实,通过免疫组织化学方法和逆转录聚合酶链反应技术检测TAK1蛋白和mRNA在子宫肌瘤及瘤旁组织中的表达量,并比较单发性和多发性肌瘤组织中以及不同直径的子宫肌瘤组织中TAK1蛋白和mRNA蛋白表达是否存在差异性。结果:TAK1在子宫肌瘤组织中的阳性表达率显著高于瘤旁正常组织(P<0.05);TAK1的mRNA和蛋白表达量与子宫肌瘤的大小相关(P<0.05),与子宫肌瘤的数目无显著相关性(P>0.05);结论:TAK1可能是子宫肌瘤发病机制中的重要参与者,有可能成为子宫肌瘤治疗的新靶点。

Objective, To compare the expression of TAK1 in normal myometrium and uterine leiomyoma tissue, and to explore the relationship between these molecules and pathogenesis of uterine leiomyoma. Methods, In the current study, form- aldehyde-fixed, paraffin-embedded specimen from 76 patients with uterine leiomyoma and normal myometrium adjacent were obtained from our hospital. The expression of TAK1 was detected by immunohistochemical staining and PCR analysis. Resuits： The expression of protein and mRNA levels TAK1 in uterine leiomyoma tissues were significantly increased when compared with normal myometrium （P〈0.05）, but not related to the number of leiomyoma （P〉0.05）. Furthermore, TAK1 expression was associated with the size of uterine leiomyoma （P〈0.05）. Conclusion：The present results suggest that the abun- dant expression of TAK1 protein may have a molecular basis characteristic of leiomyomas in the human uterus,and TAK1 may be a therapeutic target for the treatment of uterine leiomyoma.