热应激后小鼠皮层NADPH-d阳性神经元计数的时程变化

Changes in time course of NADPH-d positive neuron counts in the cortex of mice after heat stress

目的:探讨急性热应激小鼠皮层NADPH-黄递酶(NADPH-d)阳性神经元计数的时程变化。方法:复制小鼠急性热应激模型,用穿梭实验法,观察小鼠热应激后6、12、24 h不同时间点学习记忆功能。采用NADPH-d组化染色法,观察小鼠热应激后6、12 h、24 h不同时间点皮层NADPH-d阳性神经元计数的时程变化。结果:(1)穿梭实验结果表明:与对照组相比,热应激组在6、12 h时错误次数(M)明显增高,潜伏期(EL)明显缩短(P<0.05)。(2)NADPH-黄递酶组织化学染色结果显示:与对照组相比,热应激组在6 h,NADPH-d阳性神经元的数目明显增加,差异有统计学意义(P<0.05)。热应激组在12 h NADPH-d阳性神经元的数目明显减少。结论:急性热应激可以对小鼠的学习记忆造成明显的损伤,而这种损伤的机制可能与NADPH-d阳性神经元表达的增加有关。

Objective： To investigate changes in time course of NADPH-d positive neuron counts in the cortex of mice after acute heat stress. Methods： Mice model after acute heat stress were established. Shuttle box test was used to observe the learning and memory function of mice at 6 h, 12 h, 24 h respectively after heat stress. NADPH-d histochemical staining test was used to observe the time course changes of NADPH-d positive neuron counts in the cortex of mice at 6 h, 12 h, 24 h respectively after heat stress. Results： （1） Shuttle box test showed that mistakes （M） in HS （heat stress） group was significantly increased while escape latency（EL） was significantly shortened at 6 h, 12 h compared with control group （P〈0. 05）. （2） NADPH-d histochemical staining test showeded that NADPH-d positive neuron counts in HS group were significantly increased at 6h compared with control group （P〈0.05）. NADPH-d positive neuron counts in HS group were significantly decreased at 12h. Conclusion.. Acute heat stress could result in obvious damages on learning and memory function of mice, which was possibly related with the increased NADPH-d positive neuron expression.