PAK6基因沉默对前列腺癌细胞放疗敏感性研究

Effects of PAK6-shRNA Silence on Radio Sensitivity of Prostate Cancer

为探讨p21活化激酶6基因沉默对雄激素依赖性前列腺癌细胞株LNCa P细胞放疗敏感性的影响。采用利用蛋白质免疫印迹技术检测筛选出的靶向PAK6的sh RNA对LNCa P细胞中PAK6蛋白表达抑制情况。并以靶向PAK6的sh RNA转染LNCa P细胞,分别给予0、1、3、5Gy单次剂量放射治疗,观察放疗对PAK6基因沉默的前列腺癌细胞的抑制作用。用CCK-8法检测前列腺癌细胞的增殖活性;流式细胞仪测定其凋亡变化。结果显示,放疗能抑制前列腺癌细胞的增殖,增加其凋亡率;接受等剂量照射的PAK6基因沉默组前列腺癌细胞增殖减缓更明显,凋亡的肿瘤细胞显著增多。由此可知,PAK6基因沉默的前列腺癌细胞对放射治疗更敏感。

To study the effects of PAK6 silencing on human prostate cancer cell line LNCaP radio sensitivity.Methods： Western blotting was adopted to detect the expressions of PAK6 proteins in I,NCaP cells trausiected by PAK6-shRNA. LNCaP cells were transfected by PAK6-shRNA through the liposome and were locally irradiated with single dose of 0,1,3,5 Gy radiation.The prostate cancer cells inhibition by radiotherapy was observed.The proliferation and apoptosis of LNCaP cells were detected by CCK-8 and ilow cytometry respectively.Radiotherapy could inhibit the proliferation of LNCaP cells and induced apoptosis in LNCaP eells.h was apparently in PAK6 silencing group than in LNCaP group irradiated by the same dose. LNCaP silenced by PAK6-shRNA are more sensitive to radiotherapy.