精氨酸酶Ⅰ基因型与慢性牙周炎、冠心病的相关性研究

Relationship of arginase Ⅰ protein genetic polymorphisms with chronic periodontitis and coronary heart disease

目的:探讨精氨酸酶Ⅰ(ArgⅠ)rs2781666 G/T基因型与中重度慢性牙周炎(MSP)、冠心病(CHD)易感性的关系。方法:收集MSP患者50例(MSP组)、CHD患者46例(CHD组)、MSP伴CHD患者42例(MSP+CHD组)与50例同族健康对照组(HC)的颊黏膜拭子,提取DNA,采用聚合酶反应-限制性片段长度多态性法(PCR-PFLP)对ArgⅠrs2781666 G/T位点的基因型进行检测,比较其在4组间检出率的差别。结果:ArgⅠrs2781666等位基因T在CHD组中的检出率显著高于健康组,在其余各组间无统计学意义。结论:ArgⅠrs2781666等位基因T可能是CHD的一个易感基因。

Objective： To investigate the association of arginase I （Arg I ） rs2781666 G/T genotype with the susceptibility of mod- erate and severe chronic periodontitis （MSP） and coronary heart disease （CHD）. Methods： DNA was obtained from buccal swabs of 50 patients with MSP, 46 with CHD, 42 with MSP and CHD, and 50 matched healthy controls. Genotypes of rs2781666 G/T was analyzed by PCR-PFLP method. The genotype distribution in the 4 groups was compared. Results： Higher representation of Arg I rs2781666 al- lele T was found in CHD group than that in the healthy control individuals（ P 〈 0.01 ） , and there was no statistical differences among the other 3 groups. Conclusion： Allele T of Arg I rs2781666 may be associated with CHD susceptibility.