黄芩苷抑制脂多糖诱导大鼠牙周炎的组织学观察

The histological observation of the inhibitory effects of baicalin in lipopolysaccharide-induced periodontitis in rats

取10只正常大鼠作为对照组(A组),用40只大鼠右上颌第一、二磨牙间牙龈局部注射脂多糖建立大鼠牙周炎模型,随机分为4组:牙周炎组(B组)、实验组(C1、C2、C3组)(n=10),实验组实验牙的龈沟内每日注射黄芩苷溶液0.2 ml(浓度分别为0.01、0.1和1.0μg/ml),牙周炎组注射等量生理盐水,连续3 d。用药后7 d处死大鼠取样,光镜下观察组织学变化。牙周炎组牙周炎症明显重于实验组,各组破骨细胞数由多至少依次为:B组、C1组、C2组、C3组、A组,组间差异有统计学意义(P<0.05)。结果表明黄芩苷可以抑制脂多糖对大鼠牙周组织的损害。

50 adult male SD rats were divided randomly into 5 groups： A, B, C1, C2, C3（n = 10）. The rats in roup A was used as a blank controls. The rats in group B and C were with LPS induced periodontitis, those in group C1, C2 and C3 received 0. 2 ml of baicalin daily injection（0. 01,0.1 and 1.0 μg/ml respectively） into the gingival sulcus of the teeth with periodontitis for 3 days. The rats were sacri- riced 7 days after treatment and periodontal tissues of the related teeth were observed by histology. In group C1, C2 and C3 the periodontal inflammation was significantly slighter than that in group B, the osteoclasts count was as following： B 〉 C1 〉 C2 〉 C3 〉 A（P 〈0.05）. The study suggests that baicalin can inhibit destructive effect of LPS to periodontal tissue of rats.