玉米弯孢叶斑病菌ATMT突变株构建及致病力分析

Construction and Pathogenicity Analysis of ATMT Mutant of Curvularia lunata

利用农杆菌介导的基因转化(Agrobacterium-mediated transformation,ATMT)技术,转化玉米弯孢叶斑病菌(Curvularia lunata),共获得转化子1 454个。对其中菌落形态、生长速率等性状有显著变化的8个突变株进行分析。通过离体叶片接种进行筛选,发现4个突变株致病性降低,2个突变株致病性增强。通过测定生长速率、产孢量及细胞壁降解酶活性发现,其中致病性减弱的突变株,其产孢量均有所下降甚至不产孢,突变株的PG酶活性普遍增强,但Cx酶活性没有明显变化,而2株强致病性突变株的Cx酶活性明显增强。

Agrobacterium-mediated transformation were used to construct the transformants of the Curvularia lunata and 1454 transformants were obtained.Then screened eight mutants in which colonial morphology,mycelial growth and the expression of disease related genes changed more distinct.Inoculating the mutants into the detached leaves has been used to screen mutant strains and found that 4 mutant strains whose pathogenicity reduced,and 2 mutant strains whose pathogenicity enhanced.Determining the growth rate,sporulation quantity and the activity of cell wall degradation enzymes showed that the sporulation quantity of the mutants whose pathogenicity reduced were all decreasd and one of the strains did not even produce a spore.The activity of the PG enzyme enhanced generally,but the changes of the Cx enzyme did not have differences apparently.However,the activity of the Cx enzyme of the two mutants whose pathogenicity enhanced was increased.