慢性阻塞性肺疾病患者呼出气冷凝液中白介素8、白介素17水平及其与第1秒用力呼气末容积占预计值百分比的关系研究

Correlation Between the Levels of IL-8 and IL-17 in Exhaled Breath Condensate and FEV_1% of Patients With Chronic Obstructive Pulmonary Disease

目的了解慢性阻塞性肺疾病(COPD)患者呼出气冷凝液(EBC)中的白介素8(IL-8)和白介素17(IL-17)水平,并探讨其与第1秒用力呼气末容积占预计值百分比(FEV1%)的相关性。方法选取2014年11月—2015年6月在石河子大学医学院第一附属医院呼吸内科住院治疗的COPD急性加重期(AECOPD)患者81例,为病例组;选取同时期在本院进行体格检查的健康成年人78例,为对照组。分别于入院时和经治疗恢复稳定期后,收集并检测两组患者EBC,同时对其进行肺功能检查。结果两组性别、平均年龄、民族及BMI比较,差异无统计学意义(P>0.05)。治疗前两组EBC中IL-8和IL-17水平比较,差异有统计学意义(P<0.05);治疗后两组EBC中IL-8和IL-17水平比较,差异有统计学意义(P<0.05);治疗后病例组EBC中IL-8和IL-17水平与治疗前比较,差异有统计学意义(P<0.05)。治疗前两组FEV1%比较,差异有统计学意义(P<0.05);治疗后两组FEV1%比较,差异有统计学意义(P<0.05);治疗后病例组FEV1%与治疗前比较,差异有统计学意义(P<0.05)。AECOPD患者EBC中IL-8(r=0.11,P>0.05)和IL-17(r=0.09,P>0.05)水平与FEV1%无直线相关关系;COPD稳定期患者EBC中IL-8(r=-0.21,P>0.05)和IL-17(r=-0.03,P>0.05)水平与FEV1%无直线相关关系;治疗前后患者EBC中IL-8(r=-0.33,P<0.05)和IL-17(r=-0.30,P<0.05)改善值与FEV1%改善值存在负相关关系。结论 COPD患者EBC中IL-8和IL-17水平改善情况与FEV1%改善情况有关,可以作为患者病情严重程度和治疗效果的评估指标。

Objective To explore the levels of IL-8 and IL-17 in exhaled breath condensate（ EBC） of patients with chronic obstructive pulmonary disease（ COPD）,and investigate their correlation with FEV1%.Methods We enrolled 81 patients with acute COPD who were admitted into the Department of Respiratory of the First Affiliated Hospital of Medical College of Shihezi University from November 2014 to June 2015 as case group,and we also enrolled 78 healthy adults who received physical examination in the hospital in the same period.At admission and in stable phrase after treatment,EBC of the two groups was collected and detected,and the pulmonary function test was conducted.Results The two groups were not significantly different in gender,average age,ethnic group and BMI（ P〈0.05）.Before treatment,the two groups were significantly different in the levels of IL-8 and IL-17 of EBC（ P〈0.05）; after treatment,the two groups were significantly different in the levels of IL-8 and IL-17 of EBC（ P〈0.05）; the levels of IL-8 and IL-17 of the case group after treatment were significantly different from those before treatment（ P〉0.05）.Before treatment,the two groups were significantly different in FEV1%（ P〈0.05）; after treatment,the two groups were significantly different in FEV1%（ P〈0.05）; FEV1% of the case group after treatment was significantly different from those before treatment（ P〈0.05）.The levels of IL-8（ r = 0.11,P〈0.05） and IL-17（ r = 0.09,P〈0.05） in EBC had no correlative with FEV1% in AECOPD patients; the levels of IL-8（ r =- 0.21,P〈0.05） and IL-17（ r =- 0.03,P〈0.05） in EBC had no correlation with FEV1% in COPD patients in stable phrase; the improved value of the levels of IL-8（ r =- 0.33,P〈0.05） and that of IL-17（ r =- 0.30,P〈0.05） in EBC after treatment had negative correlation with that of FEV1%.Conclusion The decrease of the levels of IL-8 and IL-17 in COPD patients have correlation with the decrease of FEV1%,and can be used as evaluation indexes for the severity and efficacy.