基因表达谱芯片筛选宫颈癌放疗抵抗差异表达基因

Differentially expressed genes in residual cervical cancer after radiotherapy screened by c DNA microarray

目的探讨基因表达谱芯片筛选宫颈癌放疗残留癌组织与放疗前宫颈癌组织的差异表达基因。方法收集接受根治性放疗的宫颈癌患者3例,分别获取放疗至50 Gy残留癌组织和放疗前癌组织,通过基因表达谱芯片筛选出放疗前后的差异表达基因,应用荧光定量PCR对部分基因进行验证。结果基因表达谱芯片检测显示,放疗后残留癌组织表达上调3倍以上的基因有111个,表达下调3倍以上基因有127个。这些基因主要涉及DNA损伤修复、细胞周期调控、细胞粘附、细胞凋亡及自噬、细胞能量代谢、血管生成以及细胞信号转导等功能。荧光定量PCR证实CXCL12、CD74、FGF7、COL14A1、ATM和CD44基因在放疗后表达明显上调(P<0.05),而PRC1、RAD54L基因在放疗后表达明显下调(P<0.05)。结论基因表达谱芯片能筛选出宫颈癌放疗抵抗差异表达基因,为放疗敏感性的研究提供参考。

Objective To identify the differentially expressed genes in residual cervical cancer after radiotherapy by cDNA microarray. Methods Cervical cancer tissues from 3 patients before radiation and after 50 Gy dose of radiation were tested by cDNA mieroarray to select differentially expressed genes. The microarray results were validated by quantitative real-time PCR. Results Hier-archic cluster analysis identified that 238 differentiated genes exhibited ≥ 3.0-fold change, among which 111 genes were found to be in persistent up-regulation and 127 in persistent down-regulation after 50 Gy dose of radiation. These genes were involved in cell apoptosis and antophagy, cell energy metabolism, angiogenesis, cell apoptosis, cell cycle regulation, cell signaling, DNA damage repair, cell adhesion, and etc. The expression of CXCL12, CD74, FGF7, COL14A1, CD44 and ATM genes was up-regulated after radiation（P〈 0 05）. Meanwhile, PRC1 and RAD54L were down-regulated after radiation （P 〈 0.05）. Conclusion The differentially expressed genes identified by cDNA microarray are hoped to provide a foundation for the development of new diagnostic strategies and treatment for radiation resistance in cervical cancer.