聚电解质胶囊的制备及其对葡萄糖氧化酶的包埋性能研究

Fabrication of polyelectrolyte complex capsule and encapsulated property of glucose oxidase

葡萄糖氧化酶广泛应用在食品保鲜、医学诊断和生物化学等领域,但其容易因pH和温度等环境条件的变化而失活。因此,保护葡萄糖氧化酶在严苛的环境条件下免受分解和抑制具有十分重要的意义。将葡萄糖氧化酶成功包埋入聚丙烯酸-阳离子淀粉聚电解质胶囊中,研究不同pH和温度对葡萄糖氧化酶的活性影响情况。结果表明,经聚电解质胶囊包埋后,葡萄糖氧化酶在不同pH和温度条件下的活性和稳定性均得到显著改善。

Glucose oxidase（GOD）is an oxidizing exzyme with a broad range of applications in food preservation,clinical examination and biochemistry fields.Therefore,it is necessary to protect GOD activity against high temperature and strong acid or alkali environment.Herein,Glucose oxidase was successfully encapsulated in polyacrylic acid-cationic starch polyelectrolyte complex capsules.The effect of pH and temperature on the activity of glucose oxidase was investigated in detail.The results indicated that the stability and activity of encapsulated GOD were improved in aqueous with different pH and temperature compared with free GOD.