致病性大肠埃希菌感染小鼠肾脏的基因表达谱分析

Gene expression profiles of kidney infections caused by uropathogenic Escherichia coli in mice

目的了解尿道致病性大肠埃希菌(UPEC)感染小鼠肾脏的基因表达谱差异变化,探究宿主早期免疫和炎症反应过程。方法 2013年8月分别利用UPEC132菌株和磷酸缓冲液(PBS)经尿道逆行感染小鼠各20只,感染后于4和8h处死并解剖取其肾脏,随机取两组中4和8h各2只小鼠单侧肾,提取其RNA,利用基因表达谱芯片筛选其差异表达基因,并利用京都基因与基因组百科全书(KEGG)进行信号通路分析。结果与PBS对照组相比,UPEC感染组小鼠肾脏在4和8h分别有204个和1 323个基因差异表达,KEGG通路分析共有59个差异表达基因直接与宿主细胞的6条炎症和免疫信号通路相关,包括细胞因子与其受体相互作用信号通路、抗原加工与呈递信号通路、丝裂原活化蛋白激酶(MAPK)信号通路、Toll样受体信号通路、白细胞跨内皮迁移信号通路及自然杀伤性细胞介导细胞毒作用信号通路。结论利用表达谱芯片筛选得到的59个差异表达基因成为研究宿主早期免疫防御UPEC感染的潜在目的基因,为进一步揭示宿主与UPEC相互作用机制奠定基础。

OBJECTIVE To understand the differences of gene expression profiles of uropathogenic Escherichia coli infecting mouse kidneys and investigate the early immune and inflammatory reaction process of the host.METHODS The mice were infected by UPEC132 strain and PBS via the urinary tract respectively（20 mice/group）.The kidneys of the mice were harvested at 4hand 8hpost-infection.The kidneys of two mice were randomly selected in each group after 4hand 8hpost-infection,and used for the RNA extraction.Differentially expressed genes of the kidneys were screened by gene expression profile chip,and the signal pathway was analyzed by using the KEGG pathway database.RESULTS There were 204 and 1323differentially expressed genes at 4h and 8hpost-infection in the UPEC132 group comparing with the PBS control group.There were 59 genes in them,concerning the six inflammatory and immune signaling pathways of mice,including pathways for cytokinecytokine receptor interaction,antigen processing and presentation,MAPK signaling,toll-like receptor signaling,leukocyte transendothelial migration and natural killer cell mediated cytotoxicity.CONCLUSIONThe 59 differentially expressed genes were the potential target genes in the research on early immune defense against UTI in the host,laying a foundation for the further reveal on the interaction between host and UPEC.