导流杂交基因芯片技术用于人乳头状瘤病毒感染分型的检测效果

Effect of diversion hybridization gene chip technology for genotyping detection of human papilloma virus infection

目的观察核酸分子快速导流杂交基因芯片技术(HybrMax)用于人乳头状瘤病毒(HPV)感染分型的临床检验效果,为宫颈上皮内瘤变(CIN)及宫颈癌的诊断提供依据,有效提高宫颈病变的诊断水平。方法选取2012年1月-2015年1月100例妇科患者作为试验组,另选取68名正常妇女作为对照组,两组均使用导流杂交基因芯片技术,并将两组受试人员的超薄液基细胞学检测(TCT)诊断结果进行对比,数据采用SPSS16.0软件进行统计分析。结果试验组中68例患者HPV呈阳性,阳性率为68.0%,68例HPV阳性患者中单一型HPV44例、多重型HPV 24例;试验组检出较高的HPV类型有16、58、33型,分别占48.5%、17.6%、16.2%;HybrMax技术的诊断率与患者的宫颈病情成正比,其中CINⅠ、CINⅡ、CINⅢ、宫颈癌的检出率,分别为32.0%、51.9%、80.0%、94.4%。结论核酸分子快速导流杂交基因芯片技术对于宫颈癌具有较高的敏感性,能提高临床对宫颈癌以及CIN的诊断率。

OBJECTIVE To investigate the nucleic acid molecule rapid flow-through hybridization gene chip technology（HybrMax）applied to genotyping of human papilloma virus infection,so as to provide the basis for the diagnosis of cervical intraepithelial neoplasia and cervical cancer and to effectively improve the diagnosis level of the cervical lesions.METHODS Totally 100 gynecological patients during Jan.2012 to Jan.2015 were selected as the test group and another 68 normal individuals were set as the control group.Both groups used the flow-through hybridization gene chip technology,and the results of thin liquid-based cytology were compared between the two groups of participants.Data were statistically analyzed by software SPSS16.0.RESULTS Totally 68 patients were positive for HPV in the test group,the positive rate was 68.0%,including 44 cases of single type HPV and 24 cases of multi-type HPV.The higher detection rate in the test group was seen in HPV types 16,58,and 33,accounting for 48.5%,17.6%,16.2%.Diagnosis rate by HybrMax technology was proportional to the conditions of cervical diseases,the detection rate of cervical intraepithelial neoplasia（CIN）stagesⅠ,Ⅱ,Ⅲ and the detection rate of cervical cancer was 32.0%,51.9%,80.0%,94.4% respectively.CONCLUSION The nucleic acid molecule rapid flow-through hybridization gene chip technology has high sensitivity for cervical cancer and can improve clinical diagnosis level of cervical cancer and cervical intraepithelial neoplasia.