HPLC-DAD法同时测定龙胆泻肝丸中3种主要活性成分的含量

Simultaneuos determination of three main component in Longdan Xiegan pills by HPLC-DAD

目的 确立HPLC-DAD法同时测定龙胆泻肝丸中3种活性成分的含量. 方法 PLATSILTM ODS柱（250 mm x 4. 6 mm,5 μm）;流动相为甲醇（ A）-0. 1%磷酸,梯度洗脱,流速为1 mL/min,检测波长分别为280 nm（龙胆苦苷、黄芩苷）和240 nm（栀子苷）;柱温30 ℃. 结果 龙胆苦苷、栀子苷和黄芩苷线性范围分别为0. 112-2. 016μg（r=1. 000 0）、0. 007 6-0. 136 8μg（r=0. 999 8）、0. 294-5. 292μg（r=0. 999 7）. 范围内与峰面积呈良好的线性关系,平均回收率分别为100 . 5%、99 . 9%和104 . 5%. 结论 该方法准确、简便、具有良好的重现性和稳定性,适用于龙胆泻肝丸这种药品的质量控制,提高药品的质量.

Objective To establish a method for simultaneous determination of gentiopicroside,berberine and baica-lin in Longdan Xiegan pills. Methods HPLC-DAD method was employed to determine three active ingredients in Longdan Xiegan pills simultaneously. Using PLATISILTM ODS （250 mmx 4. 6 mm,5 μm） column with methanol （A）-0. 1% phosphoric acid（B） gradient：0-5 min 70%（B）,5-45 min 70% -20%,a flow rate of 1 mL/min,detec-tion wavelength was 280 nm for gentiopicroside and baicalin,while 240 nm for geniposide,and the column temperature was 30 ℃. Results The results showed that the linear range of gentiopicroside, geniposide and baicalin was 0. 112-2. 016 μg（r=1. 000 0）,0. 007 6-0. 136 8 μg（r=0. 999 8）,0. 294-5. 292 μg（r=0. 999 7） respectively and the peak area with the range appeared a good linear relationship,The average recoveries （n=6） were 100. 5%, 99. 9% and 104. 5%. Conclusion The method is accurate,simple,reproducibility and stability,which avaliable for quality control of Longdan Xiegan pills.