三氧化二砷对不同肝癌细胞系凋亡的影响

Apotosis effect of arensic trioxide on different human hepatocarcinoma cell lines in vivo

目的观察三氧化二砷(ATO)对肝癌细胞系Hep G2和Huh-7的影响,并探讨其作用机制。方法体外培育肝癌细胞Hep G2和Huh-7,选取不同浓度(0、2、4μmol/L)的ATO进行孵育细胞,倒置显微镜观察细胞形态学改变,四甲基偶氮唑蓝(MTT)法检测ATO对两种不同肝癌细胞增殖情况的影响,流式细胞术观察细胞凋亡情况,蛋白印迹法检测细胞中Bcl-2111(Bim)的表达。结果不同浓度(0、2、4μmol/L)的ATO对两种细胞系的生长起到不同程度的抑制,且呈浓度依赖性,受抑制情况两组差异无统计学意义。ATO对两组细胞系的促凋亡作用明显,两组细胞在ATO的作用下Bim均呈高表达。结论ATO对肝癌细胞Hep G2和Huh-7具有诱导凋亡的作用。

Objective To investigate the effects of arensic trioxide （ATO） on human hepatocarcinoma cell lines HepG2 and Huh-7, and explore its mechanisms. Methods HepG2 and Huh-7 cells were cultured in vivo and incubated with different concentrations of ATO（0, 2, 4 μmol/L）. After treatment, cell morphology was observed with inverted microscope and cell proliferation was determined by MTT assay, cell apoptosis by flow cytometry with annexin V/propidium iodided staining, and Bcl-2111 （Bim） was detected by Western-blot. Results The ATO with different concentrations （0, 2, 4 μmol/L） showed different degrees inhibitory effects on growth of 2 cells and concentration-dependent, which was no significant difference between HepG2 and Huh-7 cells. The ATO showed obvious effect on promoting apoptosis, and the Bim of 2 cell lines showed high expression after ATO treatment. Conclusion It is demonstrated that ATO could be induced apoptosis effect on HepG2 and Huh-7 cells.