不同高压脉冲电场条件对小球藻破壁的影响研究

Effects of Different Conditions of Pulsed Electric Field on Electroporation Rate of Chlorella vulgaris

采用响应面法(box-behnken)对影响小球藻破壁的4个因素(处理时间、脉冲场强、脉冲频率和脉冲宽度)进行了研究。通过对试验数据进行方差分析和多项式回归,建立了拟合度良好的模型(模型显著,失拟项不显著,R^2=0.980 3,R^2_(adj)=0.957 3),发现处理时间和脉冲场强对小球藻破壁的影响达到了极显著(P<0.000 1),其他两个因素影响不显著(P>0.05)。模型预测的最高破壁率和试验验证的值分别为72.51%和71.97%±1.65%,对应的条件为处理时间68.96 min、脉冲场强15k V/cm、脉冲频率1 500 Hz、脉冲宽度6μs。固定处理时间为60 min、脉冲频率为1 500 Hz、脉冲宽度为6μs,求解破壁率为100%时的脉冲场强,为18.45 k V/cm,可作进一步研究参考。

Response surface analysis（ Box-Behnken） was carried out to investigate the effect of pulsed electric field treatment on electroporation rate of Chlorella vulgaris cells. Four factors,treatment time,electric field strength,pulsed-frequency and pulsed-width were considered. Based on ANOVA and quadratic regression of experimental data,a significantly correlated model（ P〈0. 000 1,R^2= 0. 980 3） was established,in which lack of fit was not significant and R2 adjwas 0. 957 3 which was so close to R^2. Model showed that effect of treatment time and electric field strength on electroporation rate of Chlorella vulgaris cells was significant（ P〈0. 000 1）,while that of the other two factors was not significant（ P〉0. 05）. The highest electroporation rate,of which the predicted and experimental values were 72. 51% and 71. 97% ± 1. 65%,corresponding condition in which treatment time was68. 96 min,electric field strength was 15 k V / cm,pulsed-frequency was 1 500 Hz,and pulsed-width was 6 μs.Making treatment time 60 min,pulsed-frequency 1 500 Hz,pulsed-width 6 μs,when electric field strength increases up to 18. 45 k V / cm,according the model,electroporation rate can reach 100%. It can provide theoretical guidance for further research.