宫颈上皮内瘤变术后人乳头瘤病毒持续感染与Stathmin-1表达相关性及临床意义

The associations between human papilloma viruse persistent infection and Stathmin-1 expression and the clinical significance in cervical intraepithelial neoplasia

目的探讨Stathmin-1与宫颈锥形切除术后人乳头瘤病毒（HPV）持续感染的相关性及临床意义。方法收集106例2009年1月至2013年1月因宫颈上皮内瘤变（CIN）2～3级行宫颈锥形切除术病例，将其中36例复发病例设为复发组，70例未复发病例设为对照组。免疫组织化学法检测两组宫颈锥形切除组织Stathmin-1表达情况，HPV-DNA法检测高危HPV感染情况，分析两组术后HPV感染持续情况及与复发的关系。结果复发组Stathmin-1阳性表达率及HPV持续感染率显著高于对照组[88．89％（32／36）比34．29％（24，70），83．33％（30／36）比22．86％（16／70）]，差异有统计学意义（P〈0．01）。56例Stathmin-1阳性表达病例中，42例存在HPV持续感染，46例Stathmin-1阴性表达病例中，4例存在HPV持续感染，两者呈正相关（r=0．97，P〈0．01）。复发组与对照组持续HPV感染类型比较差异无统计学意义（P〉0．05）。结论Stathmin-1阳性表达与宫颈锥形切除术后患者HPV持续感染相关，影响高级别CIN预后，为防止高级别CIN复发提供新的思路和理论依据。

Objective To explore the relationship between Stathmin-1 and human papilloma viruse （HPV） persistent infection after conization of uterine cervix, and to show the clinical significance to recurrent of cervical intraepithelial neoplasia （CIN）. Methods One hundred and six patients who were treated with eonization of uterine cervix for CIN 2 - 3 grades were enrolled. Thirty-six recurrent patients were enrolled in recurrence group, and the others were enrolled in control group. The expression of Stathmin-1 in primary CIN tissues in two groups was detected by immunohistochemistry. The HPV infection was detected by HPV-DNA test. The relationship of HPV persistent infection and recurrence was analyzed. Results The positive expression rate of HPV persistent infection and HPV persistent infection rate in recurrence group were 88.89%（32/36）, 83.33%（30/36）, in control group were 34.29%（24/70） and 22.86%（16/70）, and there were significant difference （P 〈 0.01）. Forty-two patients had HPV persistent infection in 56 patients with stathmin-1 positive expression, and 4 patients had HPV persistent infection in 46 patients Stathmin-1 negative expression. There was positive correlation （r = 0.97, P 〈 0.01）. The type of HPV persistent infection in two groups was no significant difference （P 〉 0.05）. Conclusions Stathmin-1 positive expression is related to HPV persistent infection. The two factors can affect the prognosis of high-grade CIN, and can provide new cues and theory basis for the prevention of recurrence.