Wnt3a/β-catenin信号通路调节模拟微重力诱导的骨髓间充质干细胞增殖抑制

Wnt3a/β-catenin Signaling Pathway Mediates Inhibition of Proliferation of Mesenchymal Stem Cells Induced by Modeled Microgravity

微重力环境对骨髓间充质干细胞(MSCs)的增殖行为起着重要调节作用,但其中的分子机理尚不清楚.采用平行平板旋转培养装置模拟微重力效应,考察了模拟微重力效应下MSCs增殖行为的变化以及Wnt3a/β-catenin信号通路在该过程中的作用.结果发现,模拟微重力效应明显抑制MSCs的增殖行为,下调Wnt3a mRJNA的表达,降低细胞质中游离β-catenin,减少β-catenin向细胞核转移,抑制Cyclin D1的表达.结果表明,Wnt3a/β-catenin信号通路可能介导了微重力效应诱导的MSCs增殖抑制.

Microgravity is known to play an important role in regulating the proliferation of Mes- enchymal Stem Cells （MSC）. However, information regarding the molecular mechanisms that link microgravity and MSC proliferation is still limited. In this study, using a parallel-plate clinostat to simulate microgravity effect, we examined the effect of Modeled Microgravity （MMG） on the prolif- eration of bone marrow-derived MSCs and the possible role of Wnt3a/β-catenin signaling pathway in this process. The results showed that MSCs subjected to MMG for 3 days exhibited significantly decrease of cell proliferation. Moreover, MMG weakened mRNA expression of Wnt3a, reduced unbound β-catenin in cytoplasm and subsequently inhibited the translocation of β-catenin into nucleus. Expression of Cyclin D1 at mRNA level in MSCs was also impaired after treatment with MMG. These results suggest that Wnt3a/β-catenin signaling pathway may be mediate inhibition of proliferation of MSCs induced by MMG.