基于MPA包覆的Mn掺杂ZnS量子点/米托蒽醌复合体系对DNA的检测

Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System

利用3-羟基丙酸(MPA)包覆的Mn掺杂Zn S量子点与盐酸米托蒽醌(MTX)构建了一种可简单测定DNA的磷光复合体系。该体系以MTX作为良好的电子受体,通过光诱导电子转移(PIET)原理猝灭Mn掺杂Zn S量子点的室温磷光(RTP)。当体系中加入DNA后,DNA通过静电和嵌插作用与MTX结合,形成更稳定的复合物,并使MTX从Mn掺杂Zn S量子点表面脱离,Mn掺杂Zn S量子点的RTP恢复,从而实现了对DNA的痕量检测。该体系检测DNA的线性范围为0.1~20 mg·L^(-1),检出限为0.07 mg·L^(-1)。该方法可有效避免其他共存物质的干扰并可应用于实际生物样品中DNA含量的快捷测定。

A simple phosphorescent compound system for DNA detection was established by utilizing Mn（ covered with 3-hydracrylicacid（ MPA）） doped with Zn S quantum dots and mitoxantrone. By taking MTX as good electron acceptor,the room temperature phosphorescence（ RTP） of Mn-doped Zn S quantum dots were quenched through photoinduced electron transfer principle. After the addition of DNA in system,DNA and MTX were combined through static electricity and intercalation,and a more stable compound was formed as well,which enable MTX removing from the surface of Mn-doped Zn S quantum dots and achieving RTP recovery of Mn-doped Zn S quantum dots. Thus,the trace detection of DNA was realized. The linear scope of this system for DNA detection and the detection limit is 0. 1- 20 mg · L^-1 and 0. 07 mg · L^-1,respectively. This method can effectively avoid the interference from other coexisting substances,which can be used in rapid detection of DNA content in practical biological sample.