青蒿琥酯对MRL／lpr小鼠间质性肺炎和颌下腺炎的治疗作用

Effects of artesunate on interstitial pneumonia and submaxillaritis in MRL/lpr mice

目的探讨青蒿琥酯对MRL／lpr小鼠间质性肺炎和颌下腺炎的治疗作用。方法18只MRL／lpr小鼠按随机数字表法分为硫酸羟氯喹组、青蒿琥酯组和对照组，每组6只。18周龄时，硫酸羟氯喹组和青蒿琥酯组分别给予硫酸羟氯喹混悬液（150mg／kg，1次／d）和青蒿琥酯混悬液（50mg／kg，1次／d），共12周。HE染色法评价肺炎和颌下腺炎的病理学改变。采用酶联免疫吸附法检测血清和尿单核细胞趋化蛋白-1（monocyte chemoattractant protein-1, MCP-1）水平。结果30周龄时，硫酸羟氯喹组、青蒿琥酯组细支气管周围损害指数[（1．62±0．19）、（1．52±0．30）比（1．95±0．34）：P〈0．05]、血管周围损害指数[（1．23±0．18）、（1．28±0．12）比（1．57±0．33）；P〈0．05]、肺泡损害指数[（1．35±0．16）、（1．37±0．10）比（1．72±0．34）；P〈0．05]、颌下腺炎症指数[（1．48±0．22）、（1．43±0．15）比（1．84±0．34）：P〈0．05]均较对照组减轻，血清[（1103．02±185．56）pg／ml、（1072．37±242．43）pg／ml比（1490．67±329．43）pg／ml；P〈0．05]和尿[（189．16±70．85）pg／ml、（198．79±113．47）pg／ml比（446．79±192．31）pg／ml；P〈0．05]MCP-1水平均显著降低。结论青蒿琥酯可降低MRL／lpr小鼠血清MCP-1水平，减轻间质性肺炎和下颌腺炎。

Objective To investigate the effects of artesunate （ART） on interstitial pneumonia and sialadenitis in MRL/lpr mice. Methods A total of 18 MRL/lpr mice were randomly allocated to a hydroxychloroquine sulfate （HCQ） group, a ART group and a control group. At the age of 18 weeks, the mice in the HCQ group and ART group were given HCQ 150 mg/kg daily and ART 50 mg/kg daily for 12 weeks, respectively. The histopathological changes of pneumonitis and subrnaxillaritis were assessed by hematoxylin and eosin staining. The levels of rnonocyte chernoattraetant protein-1 （MCP-1） in the serum and urine were measured by the enzyme-linked immunosorbent assay. Results At the age of 30 weeks, the index of peribronchiolar lesion （1.62 ± 0.19, 1.52 ± 0.30 vs. 1.95 ± 0.34; all P〈0.05）, the index of perivascular lesion （1.23 ± 0.18, 1.28 ± 0.12 vs. 1.57 ± 0.33; all P〈0.05）, the alveolar lesions index （1.35 ± 0.16, 1.05 ± 0.15 vs. 1.72 ± 0.34; all P〈0.05） and the submaxillaritis index （1.48 ± 0.22, 1.43 ± 0.15 vs. 1.84 ± 0.34; all P〈0.05） in the HCQ group and the ART group were significantly decreased than those in the control group. The MCP-1 levels in the serum （1 103.02 ± 185.56 pg/rnl, 1 072.37 ± 242.43 pg/rnl vs. 1 490.67 ± 329.43 pg/ml; all P〈0.05） and urine （189.16 ± 70.85 pg/ml, 198.79 ± 113.47 pg/ml vs. 446.79 ± 192.31 pg/ml; all P〈0.05） in the HCQ group and the ART group were significantly lower than those in the control group. Conclusion ART can decrease the MCP-1 level, and ameliorate interstitial pneumonitis and sialadenitis in MRL/Ipr mice.