柑橘属药材乙酸乙酯提取物抑制黄嘌呤氧化酶作用的谱效关系研究

Spectrum-effect relationship of ethyl acetate extracts from Chinese materia medica in Citrus on xanthine oxidase inhibitory activity

目的阐明不同柑橘属药材乙酸乙酯提取物指纹图谱特征峰对黄嘌呤氧化酶抑制作用的贡献程度。方法建立枳实、青皮、陈皮、橘红和香橼乙酸乙酯提取物指纹图谱，HPLC法测定黄嘌呤氧化酶一黄嘌呤反应体系尿酸生成量，评价各提取物对黄嘌呤氧化酶活性的影响，采用灰关联度分析法研究谱效关系，对照品比对法鉴定主要色谱峰。结果谱效关系分析表明，各药材提取物的黄嘌呤氧化酶抑制活性是多成分共同作用的结果，各色谱峰所代表的化学成分对抑制黄嘌呤氧化酶活性的贡献大小顺序为圣草次苷〉柚皮苷〉柚皮素〉橙皮苷〉桔皮素〉川陈皮素〉新橙皮苷〉橙皮素。结论不同柑橘属药材乙酸乙酯提取物指纹图谱特征峰与黄嘌呤氧化酶抑制作用有一定的对应关系。

Objective To explore the fingerprint characteristic peaks contribute to xanthine oxidase inhibitory activity of ethyl acetate extracts from medicinal and edible Chinese materia medica in Citrus, and to characterize the material basis. Methods The HPLC fingerprints of ethyl acetate extracts from Aurantii Fructus Immaturus, Citri Reticulatae Pericarpium Viride, Citri Reticulatae Pericarpium, Citri Exocarpium Rubrum, and Citri Fructus were established, and the degree of enzyme inhibition was calculated by measuring the formation amount of uric acid in the xanthine oxidase-xanthine reaction system. The spectrum-effect relationship was analyzed by grey relation analysis. The mainly characteristic peaks were identified by comparision with reference standards. Results The xanthine oxidase inhibitory activity was resulted from the combined action of multiple components. The contribution order was as follows： eriocitrin, naringin, naringenin, hesperidin, tangeretin, nobiletin, neohesperidin, and hesperetin, respectively. Conclusion The relationships between the fingerprint characteristic peaks of ethyl acetate extracts from Chinese materia medica in Citrus and xanthine oxidase inhibitory activity are paralleled to some extent.