聚乙二醇a干扰素对慢性乙型肝炎患者血清IFN-γ、IL-17、IL-10的影响作用及机制

Effect of treatment with PEGIFNa on serum levels of IFN-γ, IL-17 and IL-10 in patients with chronic hepatitis B

目的:研究干扰素-g(interferon-g,IFN-γ)、白介素(interleukin,IL)-17与IL-10在慢性乙型肝炎发病中的作用机制,观察聚乙二醇a干扰素(polyethylene glycol interferona,PEG-IFNa)抗病毒治疗对上述细胞因子水平的影响.方法:应用PEG-IFNa治疗的不同时间点抽取慢性乙型肝炎患者及健康对照组静脉血,分离血清,检测血清中乙型肝炎表面抗原(hepatitis B surface antigen,HBsA g)、乙型肝炎病毒脱氧核糖核酸定量(HBV DNA)、谷丙转氨酶(alanine aminotransferase,ALT)以及血清中的IFN-γ、IL-17、IL-10.结果:慢性乙型肝炎患者IFN-γ、IL-17、IL-10的水平均显著高于健康对照组(P<0.01).抗病毒治疗后,慢性乙型肝炎患者血清IFN-γ、IL-17和IL-10的水平均较治疗前显著性下降(P<0.05).结论:IFN-γ、IL-17、IL-10共同参与了慢性乙型肝炎的发病,此结论对预测抗病毒疗效有指导意义.

AIM： To detect serum levels of interferon-g（IFN-γ）, interleukin（IL）-17 and IL-10 in patients with chronic hepatitis B after treatment with PEG-IFNa. METHODS： Serum samples were collected from patients with chronic hepatitis B, and hepatitis B surface antigen（HBsA g）, HBV DNA, alanine aminotransferase（ALT）, IFN-γ, IL-17 and IL-10 were tested. RESULTS： Serum levels of IFN-γ, IL-17 and IL-10 in patients with chronic hepatitis B were signif icantly higher than those in normal controls（P 0.01）. Treatment with PEG-IFNa signif icantly decreased serum levels of IFN-γ, IL-17 and IL-10（P 0.05）. CONCLUSION： IFN-γ, IL-17 and IL-10 may participate in the pathogenesis of chronic hepatitis B and may have a correlation with histological inflammation. These indicators may be used to assess the result of antiviral treatment.