微小RNA-152对肝癌细胞HepB3与SNU449生物学功能的影响

The Influence of miRNA-152 on Biological Function of Hepatoma Cells HepB3 and SNU449

目的:研究微小RNA-152(miR-152)对肝癌细胞生物学功能的影响。方法:将miR-152阻遏物及模拟物转染至HCC细胞系HepB3及SNU449中,检测HCC细胞生长、凋亡及Caspase3/7活性的变化。结果:miR-152阻遏物可抑制HCC细胞生长、诱发凋亡及促进Caspase3/7活性的增加。结论:miR-152在肝癌的发展中发挥重要作用,并有望成为HCC的新的治疗靶标。

Objective:The Influence of miRNA-152 on Biological Function of Hepatoma Cells.Methods:Transfer miR-152 repressor and mimics to HCC cells HepB3 and SNU449,examine the growth and apoptosis of HCC cells and the change of activity of Caspase3/7.Results:miR-152 repressor can repress the growth of HCC cells,induce apoptosis and facilitate the activity of Caspase3/7.Conclusion:miR-152 has great influence on the development of liver cancer and is promising to become the new therapeutic target of HCC.