冰片联合灯盏花素对缺氧/复氧损伤血脑屏障通透性的影响

Effects of Borneolum Syntheticum Combined with Breviscapine on Blood-brain Barrier Permeability Induced by Hypoxia/Reoxygenation Injury

目的观察冰片联合灯盏花素对缺氧/复氧下血脑屏障(BBB)通透性的影响,探讨其保护BBB的作用机制。方法培养原代大鼠脑微血管内皮细胞(BMEC),在缺氧培养箱中缺氧2 h,再复氧24 h,建立单层BMEC体外BBB模型。实验分为正常对照组、模型组、冰片组、灯盏花素组、冰片+灯盏花素组,各给药组给予相应药物干预,检测各组跨内皮细胞电阻(TEER)值和辣根过氧化物酶(HRP)的通透性,ELISA、Western blot检测BMEC中紧密连接蛋白ZO-1及Claudin-5蛋白表达。结果缺氧/复氧损伤导致TEER值降低,HRP通透率增加,ZO-1及Claudin-5蛋白表达降低;冰片联合灯盏花素可明显改善上述损伤。结论冰片联合灯盏花素对缺氧/复氧下BBB的损伤具有保护作用,其机制可能与上调ZO-1、Claudin-5蛋白表达有关。

Objective To observe effects of Borneolum Syntheticum combined with breviscapine on blood-brain barrier(BBB) permeability induced by hypoxia/reoxygenation injury. Methods The BBB model in vitro was established by primarily culturing brain microvascular endothelial cells(BMEC), hypoxia 2 h and reoxygenation 24 h. The experiment was divided into normal control group, model group, Borneolum Syntheticum group, breviscapine group, and Borneolum Syntheticum-breviscapine group. All treatment groups were given relevant medicine. TEER and HRP were detected; ELISA and Western blot was used to detect the expressions of ZO-1 and Claudin-5 in BMEC. Results Hypoxia/reoxygenation induced injury decreased TEER, increased permeability rate of HRP, and decreased the expressions of ZO-1 and Claudin-5; while Borneolum Syntheticum combined with breviscapine could improve damage caused by hypoxia/reoxygenation. Conclusion Borneolum Syntheticum combined with breviscapine shows obvious protective effect on BBB permeability induced by hypoxia/reoxygenation, and the mechanism is involved in the regulation of ZO-1 and Claudin-5 protein expressions.