响应面法优化桑椹黄酮超声辅助提取工艺及对酪氨酸酶活性抑制研究

Optimization of Ultrasonic-assisted Extraction Technology of Flavonoids from Mori Fructus by Response Surface Methodology and Study on Anti-tyrosinase Activity

目的优选桑椹黄酮的提取工艺及其对酪氨酸酶活性的抑制。方法在单因素试验基础上,采用响应面Box-Behnken中心组合设计试验,以乙醇浓度、料液比、超声时间、超声温度为自变量,黄酮含量为响应值,建立二次回归方程,研究各因素对黄酮含量的影响,同时运用多巴速率氧化法测定提取液对酪氨酸酶活性的影响。结果通过二次回归模型响应面分析确定了超声辅助提取桑椹黄酮的最佳条件:40%乙醇,料液比1∶30,超声温度60℃,超声时间50 min。在此条件下,黄酮含量为(19.16±0.23)mg/g,与理论预测值吻合,酪氨酸酶抑制率为(61.47±1.79)%。结论由响应面法优化得到的桑椹黄酮提取工艺方便可行,且具有较强的酪氨酸酶活性抑制作用。

Objective To optimize the extraction technology of flavonoids from Mori Fructus and study its anti-tyrosinase activity. Methods Response surface methodology Box-Behnken center combination was adopted to design the experiment based on the results of the single-factor experiments. Ethanol concentration, solid-liquid ratio, ultrasonic temperature and time were set as independent variables and flavonoid content was set as response value to establish a quadratic regression model. In addition, the effects of the extract on tyrosinase were investigated by using the rate of dopamine oxidation method. Results The optimum ultrasonic-assisted extraction technology was confirmed by response surface of quadratic regression model: ethanol concentration of 40%, solid-liquid ratio of 1:30, ultrasonic temperature of 60 ℃, and ultrasonic time of 50 min. Under these conditions, the actual contents of total flavonoids were(19.16±0.23)mg/g, which were in accordance with the theoretically predicted values. And its inhibition rate of tyrosinase activity was(61.47±1.79)%. Conclusion The extraction technology of flavonoids from Mori Fructus by response surface methodology is convenient and feasible, and has good effect of anti-tyrosinase activity.