心钠肽、脑钠肽与心房颤动的相关性研究

Association of atrial natriuretic peptide and brain natriuretic peptide with atrial fibrillation

目的：通过检测房颤患者血浆中的心钠肽（ANP）、脑钠肽（BNP）水平以及ANP基因rs5065、BNP基因rs198389单核苷酸多态性,从而探讨其与房颤的关系。方法：选取房颤患者60例为房颤组,非房颤患者30例为对照组。采用酶联免疫吸附法（ELISA）测定血浆心钠肽、脑钠肽水平,采用Sanger测序法进行rs5065、rs198389的DNA检测。结果：房颤组血浆心钠肽水平明显高于对照组[（982.24±139.44）pg/m L vs（884.42±136.79）pg/m L];房颤组血浆脑钠肽水平明显高于对照组[（160.14±24.28）pg/m L vs（129.00±27.11）pg/m L]（P〈0.01）。ANP rs5065位点的基因型频率在房颤组及对照组无差别（AA vs AG＋GG,P〉0.05）,rs5065的A、G等位基因在房颤组与对照组之间均无统计学差异。rs198389位点的基因型频率在房颤组及对照组无差别（AA vs AG＋GG,P〉0.05）。rs198389的A、G等位基因在两组间也无统计学差异。BNP的水平的增高与房颤发生的风险存在关联（OR=1.051,95%CI=1.020~1.083,P=0.001）。结论：房颤患者血浆ANP、BNP水平显著高于对照组。rs5065、rs198389位点单核苷酸多态性与房颤发生的风险无显著相关性。BNP水平的增高与房颤发生的风险存在关联。

Objective： To determine the plasma level of atrial natriuretic peptide（ ANP） and brain natriuretic peptide（ BNP） as well as single nucleotide polymorphism（ SNP） of ANP gene rs5065 and BNP gene rs198389 for clarifying the association of these peptides with incidence of atrial fibrillation（ AF）.Methods： Sixty patients with AF were included as AF group and another 30 subjects without AF as controls. Enzyme-linked immunosorbent assay（ ELISA）was used to measure the plasma ANP and BNP concentration,and Sanger sequencing was performed to determine minor alleles of rs5065 and rs198389.Results： Higher ANP and BNP levels were observed in AF group[ANP：（ 982. 24 ± 139. 44） pg / m L vs（ 884. 42 ± 136. 79） pg / m L; BNP：（ 160. 14 ±24. 28） pg / m L vs（ 129. 00 ± 27. 11） pg / m L,respectively,P〈0. 01]. There was no difference between AF group and control group regarding the ANPrs5065（ AA vs AG ＋ GG,P〈0. 05） and rs198389 genotype frequencies（ AA vs. AG ＋ GG,P〈0. 05）,and the difference was not statistically significant concerning A and G alleles at rs5065 and rs198389 in the two groups. However,incidence of AF was increased with elevated BNP levels（ OR =1. 051,95% CI = 1. 020- 1. 083,P = 0. 001）. Conclusion： Patients complicated with AF have higher plasma ANP and BNP concentration,and the minor alleles of rs5065 and rs198389 are not risk genes for AF,yet increased BNP level may be risks associated with incidence of AF.