水稻抗瘟基因Pi-b与Pi-5的分子检测及外引抗源的利用评价

Molecular Detection of Blast Resistance Genes Pi-b and Pi-5 in Northeast Rice Varieties and Utilization Evaluation of Introduced Resistant Donors

为了解2个广谱抗稻瘟病基因Pi-b、Pi-5在东北三省水稻品种中的分布以及部分外引材料在黑龙江省的应用价值,以68份水稻品种(52份东北三省材料、16份外引材料)和31份带有不同抗性基因的水稻单基因系为试验材料,利用基于水稻抗稻瘟病基因Pi-b、Pi-5设计的功能标记对其进行检测,同时采用黑龙江省稻瘟病优势生理小种进行混合接种鉴定。结果表明,供试材料中阳性对照BL1和沈农265、辽粳371、辽粳9号、辽星1号、中辽9052、吉粳53、吉粳94、垦稻19、垦糯2号含有抗性基因Pi-b;阳性对照Tetep和盐粳68、吉粳53、关东107、吉玉粳、九稻44、空育163、龙粳37、中156含有Pi-5基因,其中吉粳53同时含有这2个基因。接菌鉴定表明Pi-b、Pi-5 2个基因的抗性在黑龙江省无效。16份外引材料及31份水稻单基因系的接菌鉴定表明,目前对黑龙江省利用价值较高的抗瘟基因有Pi-3、Pi-7(t)、Pi-9(t)、Pi-12(t)、Pi-20、Pi-ta^2、Pi-z^t、Pi-24、Pi-25、Pi-gm。本研究对东北水稻生产中抗稻瘟病品种的选择以及抗稻瘟病育种实践具有重要意义。

To study the distribution and utilization of Pi- b and Pi- 5 in rice varieties of northeast China. 68 rice accessions,including 52 collected from Heilongjiang,Jilin and Liaoning and 16 introduced materials,and 31 rice lines with different blast resistance genes were used as experimental materials in this study. Pi- b and Pi- 5 of the 68 rice materials were detected by functional markers of Pi- b and Pi- 5 and inoculated by the dominant physiological races in Heilongjiang Province. Results showed that positive control BL1 and nine cultivars： Shennong 265,Liaojing 371,Liaojing 9,Liaoxing 1,Zhongliao 9052,Jijing 53,Jijing 94,Kendao 19 and Kennuo 2 contained the Pi- b gene,the positive control Tetep and eight cultivars ： Yanjing 68,Jijing 53,Guandong 107,Jiyujing,Jiudao 44,Kongyu 163,Longjing 37,Zhong 156 contained the Pi- 5 gene. In which Jijing 53 contained both two genes. The inoculation identification revealed that Pi- b and Pi- 5 were invalid in Heilongjiang province. By the inoculation identification of16 introduced materials and 31 rice varieties with different blast resistance genes,some blast resistance genes such as Pi- 3、Pi- 7（ t） 、Pi- 9（ t） 、Pi- 12（ t） 、Pi- 20、Pi- ta2、Pi- zt、Pi- 24、Pi- 25、Pi- gm showed potential utilization value. The results was of greatimportance in the rice production and rice blast resistance breeding in the northeast of China.