花生中低温胁迫相关转录因子基因的筛选

Profiling of Genes Encoding Cold Stress-related Transcription Factors in Peanut

为了研究花生低温胁迫下的基因表达调控机理,以花生花育19为材料,通过低温处理后芯片杂交实验,筛选花生叶片中低温胁迫响应转录因子基因。结果表明,175个具有转录调控活性的基因在低温胁迫的花生叶片中表达变化量达到2倍以上,其中92个为上调基因,83个为下调基因。通过基因功能分类分析发现,这些基因中53个上调基因和46个下调基因编码转录因子。进一步分析发现,参与花生低温抗性调控的转录因子主要包括MYB、WRKY、NAC及AP2/ERF等家族蛋白。此外,一些不包含已知保守结构域的转录因子也参与了花生低温抗性调控。本研究为花生低温抗性调控研究提供了新的转录因子基因资源。

In this article,transcription factor genes involved in cold stress regulation were screened using microarray hybridization method to study the regulatory mechanism of gene expression in the leaves of peanut（ Arachis hypogaea L.cultivar Huayu19）. The results indicated that the expression of 175 genes in peanut leaves functioning in transcription regulation had more than 2- fold changes under cold condition. Among which,92 genes were up-regulated and 83 were down-regulated. Gene functional classification analysis indicated that 53 up-regulated genes and 46 down-regulated genes encode transcription factors. Further analysis indicated that transcription factors involved in peanut cold stress regulation mainly included MYB,WRKY,NAC,AP2/ERF family members,and so on. In addition,some transcription factors involved in peanut cold stress regulation hadn＇ t known conserved domain. This study offered some candidate transcription factor genes as gene resources of tolerance to cold stress and provided theoretical basis for molecular study of abiotic stress regulation in peanut.