补益营卫方对衰老小鼠皮肤端粒酶表达的影响

The effect of telomerase expression of mice skin aging with Recipe of Supplenting Ying-wei

目的:观察补益营卫方对衰老小鼠皮肤端粒酶表达的影响,探讨补益营卫方延缓皮肤衰老的机制。方法:以自然衰老小鼠为模型,制备石蜡切片,应用免疫组化法对端粒酶的表达进行分析。结果:衰老小鼠皮肤中端粒酶的表达水平较年轻小鼠低,且有显著性差异(P<0.05)。应用补益营卫方高、中、低剂量组处理后,可显著提高端粒酶的表达,与老龄模型组比较有统计学意义(P<0.05)。三个剂量组相比,低剂量组对端粒酶表达水平的影响优于其它两个剂量组(P<0.01),中剂量组和高剂量组相比没有明显差异(P>0.05)。结论:补益营卫方通过促进衰老皮肤端粒酶的表达,起到延缓皮肤衰老作用。

Objective To observe the effect of telomerase expression of Recipe of Supplementing Ying-wei on telomerase of mice skin aging,and discuss its mechanism of delaying skin aging. Methods The natural mice skin were used as the model and preparation of paraffin section.Using Immunohistochemical technique to anylize Supplementing Ying-wei on Telomerase expression. Results The expressin of telomerase in the mice aging skin was lower than young mice,and there were significant difference（P 0.05）.Supplementing Ying-wei groups were significant improve,and compared with the aged modle group there are statistically significant（P 0.05）.Between three dose groups, the influence of low dose group＇s expression level was higher than other group（P 0.01）,middle dose group and high dose group were no significant difference（P 0.05）. Conclusion Supplementing Ying-wei can promoting expression of Telomerase of skin aging. That＇s can delay skin aging.