HPLC法测定八珍液中地黄苷A、地黄苷D、去氢土莫酸和茯苓酸

Determination of rehmaionoside A, rehmaionoside D, dehydrotumulosic acid, and pachymic acid in Bazhen Solution by HPLC

目的建立HPLC同时测定八珍液中的地黄苷A、地黄苷D、去氢土莫酸和茯苓酸的方法。方法 Kromasil C18色谱柱（200 mm×4.6 mm,5μm）;流动相：乙腈–0.05%磷酸溶液,梯度洗脱;检测波长：203 nm（0-34 min,检测地黄苷A和地黄苷D）、210 nm（34-65 min,检测去氢土莫酸和茯苓酸）;体积流量1.0 m L/min。结果地黄苷A、地黄苷D、去氢土莫酸和茯苓酸分别在5.76-115.20、4.05-81.00、6.10-122.00、6.35-127.00μg/m L线性关系良好;平均回收率分别为98.90%、99.08%、98.71%、97.95%,RSD值分别为1.49%、1.19%、1.63%、0.92%。结论该方法操作简便,准确,重复性好,可作为八珍液的质量控制方法。

Objective To establish an HPLC method for simultaneous determination of rehmaionoside A, rehmaionoside D, dehydrotumulosic acid, and pachymic acid in Bazhen Solution. Methods The determination was carried out on Kromasil C18 column（200 mm × 4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile- 0.05% phosphoric acid solution with gradient elution at a flow rate of 1.0 m L/min. The column temperature was set at 30 ℃, and the detection wavelengths were 203 nm in 0 — 34 min（determination of rehmaionoside A and rehmaionoside D） and 210 nm in 34 — 65 min（determination of dehydrotumulosic acid and pachymic acid）. Results There were good linear relationships of rehmaionoside A, rehmaionoside D, dehydrotumulosic acid, and pachymic acid in the concentration ranges of 5.76 — 115.20 μg/m L, 4.05 — 81.00 μg/m L, 6.10 — 122.00 μg/m L, and 6.35 — 127.00 μg/m L. The average recoveries were 98.90%, 99.08%, 98.71%, and 97.95% with RSD 1.49%, 1.19%, 1.63%, and 0.92%, respectively. Conclusion The method is simple, accurate, and repeated, which can be used in quantity control for Bazhen Solution.